| Title: | Rapid detection of Escherichia coli in dairy milk using static headspace-comprehensive two-dimensional gas chromatography |
| Author(s): | Heng WS; Jadhav SR; Ueland M; Shellie RA; |
| Address: | "School of Exercise and Nutrition Sciences, CASS Food Research Centre, Deakin University, 221 Burwood Highway, Burwood, Australia. Centre for Forensic Science, School of Mathematical and Physical Sciences, University of Technology Sydney, 15 Broadway, Ultimo, Australia. School of Exercise and Nutrition Sciences, CASS Food Research Centre, Deakin University, 221 Burwood Highway, Burwood, Australia. Robert.Shellie@utas.edu.au. Centre for Food Innovation, Tasmania Institute of Agriculture, University of Tasmania, Locked Bag 1325, Launceston, Australia. Robert.Shellie@utas.edu.au" |
| DOI: | 10.1007/s00216-022-04485-7 |
| ISSN/ISBN: | 1618-2650 (Electronic) 1618-2642 (Linking) |
| Abstract: | "A new approach is introduced for rapid and reliable bacteria detection in food. Namely, static headspace-comprehensive two-dimensional gas chromatography (HS-GC x GC) with backflushing. The introduced approach provides fast detection of Escherichia coli (E. coli) in enriched ultra-high-temperature processed (UHT) dairy milk. The presence of E. coli may be indicated by detecting microbial volatile organic compounds emanating from test solutions inoculated with E. coli. In the present investigation, HS-GC x GC analysis is preceded by conventional enrichment in nutrient broth and inoculated samples are clearly discernable from controls following as little as 15 h sample enrichment. Headspace equilibration for 28 min followed by an 8 min GC x GC analysis of enriched test solutions reduces time-to-response by approximately one full day compared to conventional culture-based methods. The presence of ethanol, 1-propanol, and acetaldehyde may be used as a putative marker of E. coli contamination in milk and the introduced approach is able to detect single-cell initial bacterial load. Faster, reliable detection of pathogens and/or spoilage microbes in food products is desirable for the food industry. The described approach has great potential to complement the conventional workflow and be utilised for rapid microbial screening of foodstuff" |
| Keywords: | Animals *Escherichia coli Gas Chromatography-Mass Spectrometry/methods Milk/chemistry Ethanol/analysis Bacteria *Volatile Organic Compounds/analysis Backflush Escherichia coli Food safety GC x GC Headspace Mvoc Metabolites Microbial volatile organic compo; |
| Notes: | "MedlineHeng, Wan Sin Jadhav, Snehal R Ueland, Maiken Shellie, Robert A eng Germany 2022/12/21 Anal Bioanal Chem. 2023 May; 415(13):2535-2545. doi: 10.1007/s00216-022-04485-7. Epub 2022 Dec 21" |
