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FEBS Lett

Title:		The sexual inducer of Volvox carteri. Its large-scale production and secretion by Saccharomyces cerevisiae
Author(s):		Haas E; Sumper M;
Address:		"Lehrstuhl Biochemie I, Universitat Regensburg, Germany"
Journal Title:		FEBS Lett
Year:		1991
Volume:		294
Issue:		3
Page Number:		282 - 284
DOI:		10.1016/0014-5793(91)81449-i
ISSN/ISBN:		0014-5793 (Print) 0014-5793 (Linking)
Abstract:		The DNA sequence coding for the sexual inducer glycoprotein of Volvox carteri and its N-terminal signal peptide was placed under the control of the repressible acid phosphatase promoter of the yeast Saccharomyces cerevisiae in a yeast-E. coli shuttle vector. Yeast transformed by this construct synthesized and secreted into the culture medium biologically active inducer in amounts two to three orders of magnitude higher than observed in the Volvox system
Keywords:		Animals Base Sequence DNA/chemistry/genetics Escherichia coli/genetics Eukaryota/*genetics Genetic Vectors Molecular Sequence Data Plasmids Recombinant Proteins/biosynthesis/genetics/metabolism Saccharomyces cerevisiae/genetics/*metabolism Sex Attractants;
Notes:		"MedlineHaas, E Sumper, M eng Research Support, Non-U.S. Gov't England 1991/12/09 FEBS Lett. 1991 Dec 9; 294(3):282-4. doi: 10.1016/0014-5793(91)81449-i"