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Mol Microbiol

Title:		Rgg proteins associated with internalized small hydrophobic peptides: a new quorum-sensing mechanism in streptococci
Author(s):		Fleuchot B; Gitton C; Guillot A; Vidic J; Nicolas P; Besset C; Fontaine L; Hols P; Leblond-Bourget N; Monnet V; Gardan R;
Address:		"INRA, UMR1319 MICALIS, F-78352 Jouy en Josas, France"
Journal Title:		Mol Microbiol
Year:		2011
Volume:		20110330
Issue:		4
Page Number:		1102 - 1119
DOI:		10.1111/j.1365-2958.2011.07633.x
ISSN/ISBN:		1365-2958 (Electronic) 0950-382X (Linking)
Abstract:		"We identified a genetic context encoding a transcriptional regulator of the Rgg family and a small hydrophobic peptide (SHP) in nearly all streptococci and suggested that it may be involved in a new quorum-sensing mechanism, with SHP playing the role of a pheromone. Here, we provide further support for this hypothesis by constructing a phylogenetic tree of the Rgg and Rgg-like proteins from Gram-positive bacteria and by studying the shp/rgg1358 locus of Streptococcus thermophilus LMD-9. We identified the shp1358 gene as a target of Rgg1358, and used it to confirm the existence of the steps of a quorum-sensing mechanism including secretion, maturation and reimportation of the pheromone into the cell. We used surface plasmon resonance to demonstrate interaction between the pheromone and the regulatory protein and performed electrophoretic mobility shift assays to assess binding of the transcriptional regulator to the promoter regions of its target genes. The active form of the pheromone was identified by mass spectrometry. Our findings demonstrate that the shp/rgg1358 locus encodes two components of a novel quorum-sensing mechanism involving a transcriptional regulator of the Rgg family and a SHP pheromone that is detected and reimported into the cell by the Ami oligopeptide transporter"
Keywords:		"Amino Acid Sequence Bacterial Proteins/chemistry/genetics/*metabolism DNA-Binding Proteins Electrophoretic Mobility Shift Assay Gene Expression Regulation, Bacterial Hydrophobic and Hydrophilic Interactions Mass Spectrometry Pheromones/*metabolism Phyloge;"
Notes:		"MedlineFleuchot, B Gitton, C Guillot, A Vidic, J Nicolas, P Besset, C Fontaine, L Hols, P Leblond-Bourget, N Monnet, V Gardan, R eng Research Support, Non-U.S. Gov't England 2011/03/26 Mol Microbiol. 2011 May; 80(4):1102-19. doi: 10.1111/j.1365-2958.2011.07633.x. Epub 2011 Mar 30"