| Title: | Studies in search of selective detection of isomeric biogenic hexen-1-ols and hexanal by flowing afterglow tandem mass spectrometry using [H3O]+ and [NO]+ reagent ions |
| Author(s): | Dhooghe F; Vansintjan R; Schoon N; Amelynck C; |
| Address: | "Belgian Institute for Space Aeronomy, Ringlaan 3, B-1180, Brussels, Belgium" |
| Journal Title: | Rapid Commun Mass Spectrom |
| ISSN/ISBN: | 1097-0231 (Electronic) 0951-4198 (Linking) |
| Abstract: | "RATIONALE: Plants emit a blend of oxygenated volatile C(6) compounds, known as green leaf volatiles (GLVs), in response to leaf tissue damage related to stress conditions. On-line chemical ionization mass spectrometry (CI-MS) techniques have often been used to study the dynamics of these emissions but they fail to selectively detect some important GLV compounds. METHODS: A flowing afterglow tandem mass spectrometer (FA-TMS) was used to investigate the feasibility of selective on-line detection of isomeric hexen-1-ols and hexanal. Product ions at m/z 101 and 83 from chemical ionization (CI) of these compounds by [H(3)O](+), and product ions at m/z 100, 99, 83, 82 and 72 from CI by [NO](+), have been subjected to collision-induced dissociation (CID) in the collision cell of the TMS at center-of-mass energies ranging between 0 and 9 eV. RESULTS: CID of product ions at m/z 101 and 83 from CI of GLVs with [H(3)O](+) and of product ions at m/z 83, 82 and 72 from CI of GLVs with [NO](+) resulted in identical fragmentation patterns for all measured compounds, ruling out any selectivity. However, CID of product ions at m/z 100 and 99 from CI by [NO](+) led to CID product ions with abundances differing largely between the compounds, allowing the fast selective detection of 2-hexen-1-ols, 3-hexen-1-ols and hexanal with a chosen accuracy within a well-defined range of relative concentrations. CONCLUSIONS: This research illustrates that, in contrast to common CI-MS techniques, FA-TMS allows the selective detection of hexanal in a mixture of hexanal and hexen-1-ols with a chosen accuracy for a well-defined range of relative concentrations and represents a step forward in the search for selective detection of GLVs in CI-TMS" |
| Keywords: | Aldehydes/analysis/*chemistry Hexanols/analysis/*chemistry Ions/analysis/chemistry Isomerism Nitric Oxide/chemistry Onium Compounds/chemistry Tandem Mass Spectrometry/*methods Volatile Organic Compounds/analysis/*chemistry; |
| Notes: | "MedlineDhooghe, Frederik Vansintjan, Robbe Schoon, Niels Amelynck, Crist eng Research Support, Non-U.S. Gov't England 2012/07/11 Rapid Commun Mass Spectrom. 2012 Aug 30; 26(16):1868-74. doi: 10.1002/rcm.6294" |
