| Title: | Sexual recombination as a tool for engineering industrial Penicillium chrysogenum strains |
| Author(s): | Dahlmann TA; Bohm J; Becker K; Kuck U; |
| Address: | "Lehrstuhl fur Allgemeine und Molekulare Botanik, Ruhr-University Bochum, 44780, Bochum, Germany. Lehrstuhl fur Allgemeine und Molekulare Botanik, Ruhr-University Bochum, 44780, Bochum, Germany. ulrich.kueck@rub.de" |
| DOI: | 10.1007/s00294-015-0497-7 |
| ISSN/ISBN: | 1432-0983 (Electronic) 0172-8083 (Linking) |
| Abstract: | "The recent discovery and functional characterization of opposite mating-type loci in the industrial penicillin producer Penicillium chrysogenum demonstrated their regulatory role in sexual as well as asexual development. Subsequent experiments further showed that a sexual life cycle can be induced in P. chrysogenum that was for long believed to reproduce exclusively by asexual propagation. Finally, crossing of wild type and production strains resulted in the generation of recombinant ascospore isolates. We predict from these recent findings that recombinant progeny for industrial applications can be obtained by sexual crossings and discuss experimental difficulties that occur when parental strains with karyotype heterogeneity are used for mating" |
| Keywords: | "Crosses, Genetic Fungal Proteins/*genetics/metabolism *Gene Expression Regulation, Fungal Genes, Mating Type, Fungal/genetics Genetic Heterogeneity *Industrial Microbiology Karyotype Penicillins/biosynthesis Penicillium chrysogenum/*genetics/metabolism Ph;" |
| Notes: | "MedlineDahlmann, Tim A Bohm, Julia Becker, Kordula Kuck, Ulrich eng 2015/05/23 Curr Genet. 2015 Nov; 61(4):679-83. doi: 10.1007/s00294-015-0497-7. Epub 2015 May 21" |
