| Title: | Yeast assays for G-protein-coupled receptors |
| Address: | "GlaxoSmithkline, Medicines Research Centre, Gunnels Wood Road, Stevenage, Hertfordshire, SG1 2NY, UK. sd14041@gsk.com" |
| ISSN/ISBN: | 1060-6823 (Print) 1060-6823 (Linking) |
| Abstract: | "Yeast assays for G-protein-coupled receptors have many attractions due to their simplicity, low cost, and lack of endogenous receptors. Since the first report of functional coupling of the human beta 2 adrenergic receptor to the yeast pheromone-response pathway in 1990, the technology has developed to a point at which more than 30 heterologous GPCRs are now published to couple. Major breakthroughs have come from an understanding of receptor-G protein interactions, alongside advances in knowledge of the structure of heterotrimeric G proteins. Yeast screens have been used to identify ligands both from compound collections and through the autocrine expression of peptide libraries. Yeast genetics has also been applied to a functional analysis of GPCRs and peptide ligands. In this review we describe the historical development of yeast GPCR assay systems and their current applications" |
| Keywords: | "Biochemistry/*methods Biotechnology/methods *GTP-Binding Protein alpha Subunits GTP-Binding Protein alpha Subunits, Gq-G11 *GTP-Binding Protein beta Subunits *GTP-Binding Protein gamma Subunits GTP-Binding Proteins/chemistry/*metabolism Heterotrimeric GTP;" |
| Notes: | "MedlineDowell, S J Brown, A J eng Review England 2003/04/15 Recept Channels. 2002; 8(5-6):343-52" |
