| Title: | A simple breath sampling method in intubated and mechanically ventilated critically ill patients |
| Author(s): | Bos LD; Wang Y; Weda H; Nijsen TM; Janssen AP; Knobel HH; Vink TJ; Schultz MJ; Sterk PJ; |
| Address: | "Department of Intensive Care, Academic Medical Center - University of Amsterdam, The Netherlands; Department of Respiratory Medicine, Academic Medical Center - University of Amsterdam, The Netherlands. Electronic address: 1.d.bos@amc.uva.nl. Philips Research, Eindhoven, The Netherlands. Electronic address: yuanyue.wang@philips.com. Philips Research, Eindhoven, The Netherlands. Electronic address: hans.weda@philips.com. Philips Research, Eindhoven, The Netherlands. Electronic address: tamara.nijsen@philips.com. Philips Research, Eindhoven, The Netherlands. Electronic address: apge.janssen@philips.com. Philips Research, Eindhoven, The Netherlands. Electronic address: hugo.knobel@philips.com. Philips Research, Eindhoven, The Netherlands. Electronic address: anton.vink@philips.com. Department of Intensive Care, Academic Medical Center - University of Amsterdam, The Netherlands. Electronic address: marcus.j.schultz@gmail.com. Department of Respiratory Medicine, Academic Medical Center - University of Amsterdam, The Netherlands. Electronic address: p.j.sterk@amc.uva.nl" |
| DOI: | 10.1016/j.resp.2013.11.001 |
| ISSN/ISBN: | 1878-1519 (Electronic) 1569-9048 (Linking) |
| Abstract: | "Volatile organic compounds (VOCs) in breath may serve as biomarkers of pulmonary infection or inflammation. We developed and validated a new breath sampling method for VOC analysis in ventilated patients. Breath was collected from the ventilatory circuit using cheap disposables. VOCs were identified by gas-chromatography and mass-spectrometry (GC-MS) at various minute volumes during ventilation of an artificial lung (in vitro) and ventilated patients (in vivo). Sixty-four VOCs emendated from the ventilator and tubing. Their concentrations had an inverse correlation with minute volume in in vitro experiments (median correlation coefficient: -0.61 [25-75th percentile: -0.66 to -0.43]). Forty-four of these 'ventilator-associated VOCs' were also observed in vivo, without correlations with minute volume. In vivo experiments showed that only positive end-expiratory pressure influenced the concentration of breath VOCs. The sampling method was highly reproducible (median intra-class correlation 0.95 [25-75th percentile: 0.87-0.97]). In conclusion, a novel, simple and repeatable sampling method was developed and validated for capturing exhaled VOCs in ventilated patients, which could allow for large-scale breath analysis in clinical studies" |
| Keywords: | "Aged Biomarkers Breath Tests/*methods *Critical Illness Female Gas Chromatography-Mass Spectrometry Humans Intensive Care Units Male Middle Aged Reproducibility of Results *Respiration Respiration, Artificial/instrumentation/*methods *Volatile Organic Com;" |
| Notes: | "MedlineBos, Lieuwe D J Wang, Yuanyue Weda, Hans Nijsen, Tamara M E Janssen, Anton P G E Knobel, Hugo H Vink, Teunis J Schultz, Marcus J Sterk, Peter J eng Research Support, Non-U.S. Gov't Netherlands 2013/11/19 Respir Physiol Neurobiol. 2014 Jan 15; 191:67-74. doi: 10.1016/j.resp.2013.11.001. Epub 2013 Nov 12" |
