| Title: | Rapid headspace solid-phase microextraction sheets with direct analysis in real time mass spectrometry (SPMESH-DART-MS) of derivatized volatile phenols in grape juices and wines |
| Address: | "Department of Food Science, Cornell University, 251 Stocking Hall, Ithaca, NY, USA. Department of Food Science, Cornell University, 251 Stocking Hall, Ithaca, NY, USA. Electronic address: gls9@cornell.edu" |
| DOI: | 10.1016/j.aca.2023.341577 |
| ISSN/ISBN: | 1873-4324 (Electronic) 0003-2670 (Linking) |
| Abstract: | "Volatile phenols possess 'smoky, spicy' aromas and are routinely measured in grapes, wines and other foodstuffs for quality control. Routine analyses of volatile phenols rely on gas chromatography - mass spectrometry (GC-MS), but slow throughput of GC-MS can cause challenges during times of surge demand, i.e. following 'smoke taint' events involving forest fires near vineyards. Parallel extraction of headspace volatiles onto sorbent sheets (HS-SPMESH) followed by direct analysis in real time mass spectrometry (DART-MS) is a rapid alternative to conventional GC-MS approaches. However, HS-SPMESH extraction is poorly suited for lower volatility odorants, including volatile phenols. This work reports development and validation of an HS-SPMESH-DART-MS approach for five volatile phenols (4-ethylphenol, 4-ethylguiacol, guaiacol, 4-methylguaiacol, and cresols). Prior to HS-SPMESH extraction, volatile phenols were acetylated to facilitate their extraction. A unique feature of this work was the use of d(6)-Ac(2)O as a derivatizing agent to overcome issues with isobaric interferences inherent to chromatography-free MS techniques. The use of alkaline conditions during derivatization resulted in cumulative measurement of both free and bound forms of volatile phenols. The validated HS-SPMESH-DART-MS method achieved a throughput of 24 samples in approximately 60 min (including derivatization and extraction time) with low limits of detection (<1 mug L(-1)) and good repeatability (3-6% RSD) in grape and wine matrices. Validation experiments with smoke-tainted grape samples indicated good correlation between total (free + bound) volatile phenols measured by HS-SPMESH-DART-MS and a gold standard GC-MS method" |
| Keywords: | *Vitis/chemistry *Wine/analysis Mass Spectrometry/methods Phenols/analysis Solid Phase Microextraction/methods Smoke/analysis *Volatile Organic Compounds/analysis Acetylation Ambient-ionization Smoke taint Volatile phenols Wine; |
| Notes: | "MedlineBates, Terry L Sacks, Gavin L eng Netherlands 2023/08/01 Anal Chim Acta. 2023 Sep 22; 1275:341577. doi: 10.1016/j.aca.2023.341577. Epub 2023 Jun 29" |
