| Title: | Malodorous volatile organic compounds (MVOCs) formation after dewatering of wastewater sludge: Correlation with the extracellular polymeric substances (EPS) and microbial communities |
| Author(s): | Zhu X; Yang X; Gao W; Zhao S; Zhang W; Yu P; Wang D; |
| Address: | "State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China; Yangtze River Delta Research Center for Eco-Environmental Sciences, Yiwu 322000, China. State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; Yangtze River Delta Research Center for Eco-Environmental Sciences, Yiwu 322000, China. Electronic address: xfyang@rcees.ac.cn. Research and Development Center, Beijing Drainage Group Co., Ltd, Beijing 100124, China. Hubei Key Laboratory of Yangtze Catchment Environmental Aquatic Science, School of Environmental Studies, China University of Geosciences, Wuhan 430074, Hubei, China. Department of Environment Engineering, Zhejiang University, Hangzhou 310058, China. Yangtze River Delta Research Center for Eco-Environmental Sciences, Yiwu 322000, China; Hubei Key Laboratory of Yangtze Catchment Environmental Aquatic Science, School of Environmental Studies, China University of Geosciences, Wuhan 430074, Hubei, China; Department of Environment Engineering, Zhejiang University, Hangzhou 310058, China" |
| DOI: | 10.1016/j.scitotenv.2023.161491 |
| ISSN/ISBN: | 1879-1026 (Electronic) 0048-9697 (Linking) |
| Abstract: | "Malodorous volatile organic compounds (MVOCs) are often the key odorants in determining sludge odor character and odor impact. However, the emission characterization and generation mechanisms of MVOCs from various dewatered sludge have not been sufficiently understood partly due to the diverse and complex composition and low concentration of odorants. In this study, waste activated sludge (WAS) was collected to examine the variation of MVOC emission from sludge after different dewatering treatment in lab-scale trials. The MVOCs were measured using the electronic nose (eNose), headspace gas chromatography-coupled ion mobility spectrometry (HS-GC-IMS), and headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS). The results showed that centrifugation treatment promoted the generation of various odorous volatiles. The identified key odorants included dimethyl sulfide (DMS), dimethyl disulfide (DMDS), dimethyl trisulfide (DMTS), geosmin, and p-cresol according to their odor activity values (OAVs). The effects of the enhanced dewatering on volatile production were greater than thickening, however, the key odorants of dewatered sludge using gravity thickening varied more greatly than sludges from centrifuge thickening. The distribution of extracellular polymeric substances (EPS) and variation of microbial community showed correlations with the production of key odorants. Tryptophan-like substances in the inner layer of EPS (LB-EPS and TB-EPS) were better correlated with the key odorants. The bound EPS released by centrifugation may play the role of precursor for odorous microbial volatiles. According to the predicted functions of differential microbial genera, Desulfobulbus (Desulfobacterota), Gordonia (Actinobacteriota), and Hyphomicrobium (Proteobacteria) were associated with the production of DMS, DMDS, and DMTS, while Gordonia and Hyphomicrobium were related to p-cresol production" |
| Keywords: | Wastewater Sewage/chemistry *Volatile Organic Compounds/analysis Extracellular Polymeric Substance Matrix/chemistry Gas Chromatography-Mass Spectrometry/methods Odorants/analysis *Microbiota 16s rRNA sequencing Centrifugation dewatering Hs-gc-ims Hs-spme-; |
| Notes: | "MedlineZhu, Xinmeng Yang, Xiaofang Gao, Wei Zhao, Shan Zhang, Weijun Yu, Pingfeng Wang, Dongsheng eng Netherlands 2023/01/13 Sci Total Environ. 2023 Apr 1; 867:161491. doi: 10.1016/j.scitotenv.2023.161491. Epub 2023 Jan 10" |
