| Title: | l-Arginine Inhibits Apoptosis of Ovine Intestinal Epithelial Cells through the l-Arginine-Nitric Oxide Pathway |
| Author(s): | Zhang H; Zhao F; Peng A; Guo S; Wang M; Elsabagh M; Loor JJ; Wang H; |
| Address: | "Laboratory of Metabolic Manipulation of Herbivorous Animal Nutrition, College of Animal Science and Technology, Yangzhou University, Yangzhou, PR China. Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, PR China. Department of Animal Production and Technology, Faculty of Agricultural Sciences and Technologies, Nigde Omer Halisdemir University, Nigde, Turkey. Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt. Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana, Illinois 61801, USA" |
| ISSN/ISBN: | 1541-6100 (Electronic) 0022-3166 (Linking) |
| Abstract: | "BACKGROUND: In nonruminants, many of the biological roles of l-arginine (Arg) at the intestinal level are mediated through the Arg-nitric oxide (Arg-NO) pathway. Whether the Arg-NO pathway is involved in controlling the immune response and viability in ovine intestinal epithelial cells (IOECs) is unclear. OBJECTIVES: The current study aimed to examine the role of the Arg-NO pathway in apoptosis, antioxidant capacity, and mitochondrial function of IOECs. METHODS: The IOECs were incubated in Arg-free DMEM supplemented with 150 muM Arg (CON) or 300 muM Arg (ARG) alone or with 350 muM Nw-nitro-l-arginine methyl ester hydrochloride (l-NAME) (CON + NAME, ARG + NAME) for 24 h. The reactive oxygen species (ROS) concentration, antioxidant capacity, and cell apoptotic percentage were determined. RESULTS: Arg supplementation decreased (P < 0.05) the ROS concentration (38.9% and 22.7%) and apoptotic cell percentage (57.2% and 54.8%) relative to the CON and CON + NAME groups, respectively. Relative to the CON and ARG treatments, the l-NAME administration decreased (P < 0.05) the mRNA abundance of superoxide dismutase 2 (32% and 21.3%, respectively) and epithelial NO synthase (36% and 29.1%, respectively). Arg supplementation decreased (P < 0.05) the protein abundance of apoptosis antigen 1 (FAS) (52.0% and 43.9%) but increased (P < 0.05) those of nuclear respiratory factor 1 (31.3% and 22.9%) and inducible NO synthase (35.2% and 41.8%) relative to the CON and CON + NAME groups, respectively. CONCLUSIONS: The inhibition of apoptosis in IOECs due to the increased supply of Arg is associated with the mitochondria- and FAS-dependent pathways through the activity of the Arg-NO pathway. The findings help elucidate the role of the Arg-NO pathway in IOEC growth and apoptosis" |
| Keywords: | "Animals Apoptosis Arginine/metabolism/*pharmacology Cells, Cultured Epithelial Cells/*drug effects Gene Expression Regulation/physiology Intestinal Mucosa/*cytology NG-Nitroarginine Methyl Ester/administration & dosage/pharmacology Nitric Oxide/*metabolis;" |
| Notes: | "MedlineZhang, Hao Zhao, Fangfang Peng, Along Guo, Shuang Wang, Mengzhi Elsabagh, Mabrouk Loor, Juan J Wang, Hongrong eng Research Support, Non-U.S. Gov't 2020/05/16 J Nutr. 2020 Aug 1; 150(8):2051-2060. doi: 10.1093/jn/nxaa133" |
