| Title: | N-acyl homoserine lactone binding to the CarR receptor determines quorum-sensing specificity in Erwinia |
| Author(s): | Welch M; Todd DE; Whitehead NA; McGowan SJ; Bycroft BW; Salmond GP; |
| Address: | "Department of Biochemistry, Tennis Court Road, Cambridge CB2 1QW" |
| ISSN/ISBN: | 0261-4189 (Print) 1460-2075 (Electronic) 0261-4189 (Linking) |
| Abstract: | "Quorum sensing via an N-acyl homoserine lactone (HSL) pheromone controls the biosynthesis of a carbapenem antibiotic in Erwinia carotovora. Transcription of the carbapenem biosynthetic genes is dependent on the LuxR-type activator protein, CarR. Equilibrium binding of a range of HSL molecules, which are thought to activate CarR to bind to its DNA target sequence, was examined using fluorescence quenching, DNA bandshift analysis, limited proteolysis and reporter gene assays. CarR bound the most physiologically relevant ligand, N-(3-oxohexanoyl)-L-homoserine lactone, with a stoichiometry of two molecules of ligand per dimer of protein and a dissociation constant of 1.8 microM, in good agreement with the concentration of HSL required to activate carbapenem production in vivo. In the presence of HSL, CarR formed a very high molecular weight complex with its target DNA, indicating that the ligand causes the protein to multimerize. Chemical cross-linking analysis supported this interpretation. Our data show that the ability of a given HSL to facilitate CarR binding to its target DNA sequence is directly proportional to the affinity of the HSL for the protein" |
| Keywords: | "Bacterial Proteins/chemistry/genetics/*metabolism Base Sequence Carbapenems/biosynthesis DNA Primers/genetics DNA, Bacterial/genetics/metabolism Homoserine/*analogs & derivatives/metabolism Lactones/*metabolism Ligands Pectobacterium carotovorum/genetics/;" |
| Notes: | "MedlineWelch, M Todd, D E Whitehead, N A McGowan, S J Bycroft, B W Salmond, G P eng Research Support, Non-U.S. Gov't England 2000/02/17 EMBO J. 2000 Feb 15; 19(4):631-41. doi: 10.1093/emboj/19.4.631" |
