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J Agric Food Chem

Title:		Identification of odor impact compounds of Tagetes minuta L. essential oil: comparison of two GC-olfactometry methods
Author(s):		Breme K; Tournayre P; Fernandez X; Meierhenrich UJ; Brevard H; Joulain D; Berdague JL;
Address:		"LCMBA UMR CNRS 6001, Universite de Nice-Sophia Antipolis, Parc Valrose, 06108 Nice Cedex 2, France"
Journal Title:		J Agric Food Chem
Year:		2009
Volume:		57
Issue:		18
Page Number:		8572 - 8580
DOI:		10.1021/jf9016509
ISSN/ISBN:		1520-5118 (Electronic) 0021-8561 (Linking)
Abstract:		"Odor impact compounds of Tagetes minuta L. essential oil were studied by gas chromatography (GC)-olfactometry using aroma extract dilution analysis (AEDA) and vocabulary-intensity-duration of elementary odors by sniffing (VIDEO-Sniff). AEDA was conducted by direct injection and revealed the presence of 43 odorant zones. Highest flavor dilution (FD) values were obtained for ethyl 2-methylpropanoate, ethyl 3-methylbutanoate, (E)-ocimenone, two tentatively identified thiols, and two yet unknown compounds. VIDEO-Sniff was realized by dynamic headspace sampling (D-HS) combined with 8W-GC-olfactometry where eight sniffers simultaneously detect volatile compounds obtained from a single chromatographic separation and revealed the presence of 42 odorant zones. Odorant trace compounds detected by GC-O that were present in quantities inferior to the GC-qMS system's detection limit and those subject to coelutions were identified by GC x GC-time-of-flight mass spectrometry (TOFMS). A total amount of 37 odorant components could be identified by VIDEO-Sniff, and the strong influence of the fruity notes of numerous esters stood out. Highest olfactory signals were obtained for ethyl 2-methylpropanoate, ethyl 2- and 3-methylbutanoate, and oct-1-en-3-one. Both methods hence come to the conclusion that ethyl 2-methylpropanoate and ethyl 2- and 3-methylbutanoate are among the main odorants in Tagetes minuta L. essential oil. Differences, advantages, and drawbacks of both GC-O methods are discussed"
Keywords:		"Butyrates/analysis Chromatography, Gas/*methods Gas Chromatography-Mass Spectrometry Odorants/*analysis Oils, Volatile/*chemistry Plant Oils/chemistry Propionates/analysis *Smell Tagetes/*chemistry Volatile Organic Compounds/analysis;"
Notes:		"MedlineBreme, Katharina Tournayre, Pascal Fernandez, Xavier Meierhenrich, Uwe J Brevard, Hugues Joulain, Daniel Berdague, Jean Louis eng Comparative Study Research Support, Non-U.S. Gov't 2009/08/22 J Agric Food Chem. 2009 Sep 23; 57(18):8572-80. doi: 10.1021/jf9016509"