| Title: | Generation of reactive oxygen species in cyanobacteria and green algae induced by allelochemicals of submerged macrophytes |
| Author(s): | Wang J; Zhu J; Liu S; Liu B; Gao Y; Wu Z; |
| Address: | "State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China" |
| DOI: | 10.1016/j.chemosphere.2011.06.076 |
| ISSN/ISBN: | 1879-1298 (Electronic) 0045-6535 (Linking) |
| Abstract: | "Inhibition of phytoplankton by allelochemicals released by submerged macrophytes is reported to be one of the mechanisms that maintain a clear-water state in shallow lakes. In order to elucidate this mechanism, the ability of six polyphenols and two long-chain fatty acids to induce the generation of reactive oxygen species (ROS) in phytoplankton was studied using the ROS sensitive probe 2',7'- dichlorodihydrofluorescein diacetate (DCFH-DA). The results showed that only (+)-catechin (CA) and pyrogallic acid (PA) could induce ROS formation in Microcystis aeruginosa and Pseudokirchneriella subcapitata. 25 mg L(-)(1) CA caused 1.2, 1.4 and 1.8 times increase of ROS levels in M. aeruginosa at 1, 2 and 4h exposure, respectively, and, correspondingly in P. subcapitata cells, these values were 3.7, 6.2 and 7.7, respectively. PA also significantly increased the levels of intracellular ROS in P. subcapitata (P < 0.01); however, significant ROS generation in M. aeruginosa was observed at only 4h exposure (P < 0.01). Light enhanced ROS generation in CA treated cells, but not in the cells treated with PA. CA and PA may act as redox cyclers after uptake by test organisms and produce ROS successively. These results suggest that the oxidative stress induced by the redox cycling property of allelochemicals may be one of the important causes for the inhibitory effect of some submerged macrophytes towards undesired phytoplankton in natural aquatic ecosystems" |
| Keywords: | Aquatic Organisms/*chemistry Catechin/isolation & purification/pharmacology Chlorophyta/cytology/*drug effects/metabolism *Immersion Magnoliopsida/*chemistry Microalgae/cytology/drug effects/metabolism Microcystis/cytology/*drug effects/metabolism Pheromo; |
| Notes: | "MedlineWang, Jing Zhu, Junying Liu, Shaoping Liu, Biyun Gao, Yunni Wu, Zhenbin eng Research Support, Non-U.S. Gov't England 2011/07/16 Chemosphere. 2011 Oct; 85(6):977-82. doi: 10.1016/j.chemosphere.2011.06.076. Epub 2011 Jul 14" |
