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Appl Microbiol Biotechnol

Title:		Bioconversion of green volatiles in okara (soybean residue) into esters by coupling enzyme catalysis and yeast (Lindnera saturnus) fermentation
Author(s):		Vong WC; Liu SQ;
Address:		"Food Science and Technology Programme, Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore, 117543, Singapore. Food Science and Technology Programme, Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore, 117543, Singapore. chmLsq@nus.edu.sg. National University of Singapore (Suzhou) Research Institute, No. 377 Linquan Street, Suzhou Industrial Park, Suzhou, 215123, Jiangsu, China. chmLsq@nus.edu.sg"
Journal Title:		Appl Microbiol Biotechnol
Year:		2018
Volume:		20181003
Issue:		23
Page Number:		10017 - 10026
DOI:		10.1007/s00253-018-9396-4
ISSN/ISBN:		1432-0614 (Electronic) 0175-7598 (Linking)
Abstract:		"Okara (soybean residue), a by-product from soymilk and tofu production, has a green, grassy off odour as it contains a large amount of aldehydes. This work investigated the rate-limiting enzyme(s) in the formation of aldehydes in okara and the pathways leading to their bioconversion into fruity, pleasant-smelling esters by the yeast Lindnera saturnus. Lipase and hydroperoxide lyase were shown to be rate-limiting enzymes while endogenous soy lipoxygenase was also crucial for the production of aldehydes in okara. Subsequent fermentation of okara by L. saturnus increased the amount of esters by about 70 times to 165-277 mug/g dried okara. The generation of C7 esters followed our hypothesised pathway, while that for C6 esters was mainly affected by L. saturnus. This study presents a simple and inexpensive one-pot setup for the natural bio-production of esters from okara"
Keywords:		Aldehyde-Lyases/metabolism Catalysis Cytochrome P-450 Enzyme System/metabolism Esters/*metabolism Fermentation Food Handling Industrial Microbiology Linoleic Acid/analysis Lipase/metabolism Lipoxygenase/metabolism Plant Proteins/*chemistry Polysaccharides;
Notes:		"MedlineVong, Weng Chan Liu, Shao-Quan eng R143-000-664-114/National Research Foundation Singapore/ Germany 2018/10/05 Appl Microbiol Biotechnol. 2018 Dec; 102(23):10017-10026. doi: 10.1007/s00253-018-9396-4. Epub 2018 Oct 3"