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Toxicol In Vitro

Title:		"Analysis of oxidative stress in SK-N-MC neurons exposed to styrene-7,8-oxide"
Author(s):		Vettori MV; Caglieri A; Goldoni M; Castoldi AF; Dare E; Alinovi R; Ceccatelli S; Mutti A;
Address:		"ISPESL Research Center at the University of Parma, via Gramsci 14, Parma 43100, Italy. mariavittoria.vettori@unipr.it"
Journal Title:		Toxicol In Vitro
Year:		2005
Volume:		19
Issue:		1
Page Number:		11 - 20
DOI:		10.1016/j.tiv.2004.04.015
ISSN/ISBN:		0887-2333 (Print) 0887-2333 (Linking)
Abstract:		"Styrene-7,8-oxide (SO) is the main metabolite of styrene, a neurotoxic volatile organic compound used industrially. Here we report the novel observation that several markers of oxidative stress were affected in SK-N-MC cells exposed for 16 h to concentrations of SO that induce apoptotic cell death. The production of Thiobarbituric Acid Reactive Substances (TBARS), rose from 69.1 +/- 15.7 nmol/g protein (control) to 119.3 +/- 39.2 and 102.0 +/- 17.3 nmol/g protein after exposure to 0.3 and 1 mM SO, respectively. Carbonyl group levels were significantly enhanced by SO at both concentrations. The lower dose also decreased sulphydryl groups. SO caused a marked oxidative DNA damage, as shown by a fivefold increase in 8-hydroxy-2(')-deoxyguanosine (8-OHdG). In addition, SO exposure resulted in alterations of scavenging abilities, given the reduction of both glutathione (GSH) and glutathione-S-transferase (GST) activity. Induction of expression of the oxidative stress response gene heme-oxygenase-1 (HO-1) and an increased HO-1 activity were also observed. These data provide compelling evidence that oxidative stress significantly contributes to SO toxicity in neuronal cells"
Keywords:		"8-Hydroxy-2'-Deoxyguanosine Biomarkers/metabolism Carcinogens/*toxicity Cell Line, Tumor DNA Damage Deoxyguanosine/*analogs & derivatives/metabolism Dose-Response Relationship, Drug Epoxy Compounds/*toxicity Free Radical Scavengers/metabolism Glutathione/;"
Notes:		"MedlineVettori, M V Caglieri, A Goldoni, M Castoldi, A F Dare, E Alinovi, R Ceccatelli, S Mutti, A eng Research Support, Non-U.S. Gov't England 2004/12/08 Toxicol In Vitro. 2005 Feb; 19(1):11-20. doi: 10.1016/j.tiv.2004.04.015"