| Title: | "Methanol induces cytosolic calcium variations, membrane depolarization and ethylene production in arabidopsis and tobacco" |
| Author(s): | Tran D; Dauphin A; Meimoun P; Kadono T; Nguyen HTH; Arbelet-Bonnin D; Zhao T; Errakhi R; Lehner A; Kawano T; Bouteau F; |
| Address: | "Universite Paris Diderot, Sorbonne Paris Cite, Laboratoire Interdisciplinaire des Energies de Demain, Paris, France. Department of Physiology & Cell Information Systems Group, McGill University, Montreal, Quebec, Canada. Institut Curie, CNRS UMR3215, INSERM U934, Paris, France. Sorbonne Universite, UMR7622-IBPS, Paris, France. Laboratory of Aquatic Environmental Science, Kochi University, Kochi, Japan. Graduate School of Environmental Engineering, University of Kitakyushu, Wakamatsu-ku, Kitakyushu, Japan. Eurofins Agriscience Service, Marocco. Normandie Universite, UNIROUEN, Laboratoire de Glycobiologie et Matrice Extracellulaire Vegetale, EA4358, SFR Normandie vegetal, Rouen, France. LINV Kitakyushu Research Center, Kitakyushu, Japan. Universite Paris Diderot, Sorbonne Paris Cite, Paris Interdisciplinary Energy Research Institute (PIERI), Paris, France" |
| ISSN/ISBN: | 1095-8290 (Electronic) 0305-7364 (Print) 0305-7364 (Linking) |
| Abstract: | "BACKGROUND AND AIMS: Methanol is a volatile organic compound released from plants through the action of pectin methylesterases (PMEs), which demethylesterify cell wall pectins. Plant PMEs play a role in developmental processes but also in responses to herbivory and infection by fungal or bacterial pathogens. However, molecular mechanisms that explain how methanol could affect plant defences remain poorly understood. METHODS: Using cultured cells and seedlings from Arabidopsis thaliana and tobacco BY2 expressing the apoaequorin gene, allowing quantification of cytosolic Ca2+, a reactive oxygen species (ROS) probe (CLA, Cypridina luciferin analogue) and electrophysiological techniques, we followed early plant cell responses to exogenously supplied methanol applied as a liquid or as volatile. KEY RESULTS: Methanol induces cytosolic Ca2+ variations that involve Ca2+ influx through the plasma membrane and Ca2+ release from internal stores. Our data further suggest that these Ca2+ variations could interact with different ROS and support a signalling pathway leading to well known plant responses to pathogens such as plasma membrane depolarization through anion channel regulation and ethylene synthesis. CONCLUSIONS: Methanol is not only a by-product of PME activities, and our data suggest that [Ca2+]cyt variations could participate in signalling processes induced by methanol upstream of plant defence responses" |
| Keywords: | "Aequorin/metabolism Apoproteins/metabolism Arabidopsis/drug effects/*physiology Calcium/*metabolism Cell Membrane/physiology Cells, Cultured Cytosol/metabolism Ethylenes/*metabolism Methanol/administration & dosage Plant Growth Regulators/*metabolism Plan;" |
| Notes: | "MedlineTran, Daniel Dauphin, Aurelien Meimoun, Patrice Kadono, Takashi Nguyen, Hieu T H Arbelet-Bonnin, Delphine Zhao, Tingting Errakhi, Rafik Lehner, Arnaud Kawano, Tomonori Bouteau, Francois eng Research Support, Non-U.S. Gov't England 2018/03/27 Ann Bot. 2018 Nov 3; 122(5):849-860. doi: 10.1093/aob/mcy038" |
