| Title: | Metabolomic Approach Based on Analytical Techniques for the Detection of Secondary Metabolites from Humulus lupulus L. Dried Leaves |
| Author(s): | Taiti C; Di Matteo G; Spano M; Vinciguerra V; Masi E; Mannina L; Garzoli S; |
| Address: | "Department of Agriculture, Food, Environmental and Forest, Universita di Firenze, 50019 Firenze, Italy. Department of Chemistry and Technology of Drug, Sapienza University, 00185 Rome, Italy. Department for Innovation in Biological Systems, Food and Forestry, University of Tuscia, 01100 Viterbo, Italy" |
| ISSN/ISBN: | 1422-0067 (Electronic) 1422-0067 (Linking) |
| Abstract: | "Currently, the leaves of the hop plant (Humulus lupulus L.) are an unexploited and still little-investigated agricultural by-product. In our study, with the aim of exploring the metabolome of dried hop leaves (Chinook cultivar), a metabolomic approach was applied using multiple analytical tools such as SPME/GC-MS, GC-MS, PTR-ToF-MS, and NMR to identify the secondary metabolites. The obtained results showed the presence of a high number of components belonging to different chemical classes. In fact, thanks to the multi-methodological approach, volatile organic compounds (VOCs) with low molecular weight, terpenic compounds, fatty acids, sugars, amino acids, organic acids, and alcohols have been detected and identified. Among the revealed terpenes in the untreated matrix, the sesquiterpenes alpha-humulene, beta-caryophyllene, and alpha-copaene were the most abundant. Among the saturated and unsaturated fatty acids, palmitic and linolenic acids, respectively, were those with the highest relative percentages. Particularly relevant was the sugar content, where sucrose was the main exponent while glutamate and asparagine were the principal detected amino acids. Conversely, alcohols and organic acids were the least abundant compound classes, and xanthohumol was also identified in the methanolic extract" |
| Keywords: | *Humulus Metabolomics Amino Acids Metabolome Glutamic Acid chemical composition gas chromatography-mass spectrometry nuclear magnetic resonance proton-transfer-reaction time-of-flight-mass spectrometer solid-phase microextraction volatile organic compound; |
| Notes: | "MedlineTaiti, Cosimo Di Matteo, Giacomo Spano, Mattia Vinciguerra, Vittorio Masi, Elisa Mannina, Luisa Garzoli, Stefania eng Switzerland 2023/09/28 Int J Mol Sci. 2023 Sep 6; 24(18):13732. doi: 10.3390/ijms241813732" |
