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Methods Mol Biol

Title:		Gradient Tracking by Yeast GPCRs in a Microfluidics Chamber
Author(s):		Suzuki SK; Kelley JB; Elston TC; Dohlman HG;
Address:		"Curriculum in Bioinformatics and Computational Biology, UNC School of Medicine, Chapel Hill, NC, USA. Molecular and Biomedical Sciences Department, University of Maine, Orono, ME, USA. Curriculum in Bioinformatics and Computational Biology, UNC School of Medicine, Chapel Hill, NC, USA. timothy_elston@med.unc.edu. Computational Medicine Program, UNC School of Medicine, Chapel Hill, NC, USA. timothy_elston@med.unc.edu. Department of Pharmacology, UNC School of Medicine, Chapel Hill, NC, USA. timothy_elston@med.unc.edu. Curriculum in Bioinformatics and Computational Biology, UNC School of Medicine, Chapel Hill, NC, USA. hdohlman@med.unc.edu. Department of Pharmacology, UNC School of Medicine, Chapel Hill, NC, USA. hdohlman@med.unc.edu"
Journal Title:		Methods Mol Biol
Year:		2021
Volume:		2268
Issue:
Page Number:		275 - 287
DOI:		10.1007/978-1-0716-1221-7_18
ISSN/ISBN:		1940-6029 (Electronic) 1064-3745 (Print) 1064-3745 (Linking)
Abstract:		"Cells typically exist in a highly dynamic environment, which cannot easily be recreated in culture dishes or microwell plates. Microfluidic devices can provide precise control of the time, dose, and orientation of a stimulus, while simultaneously capturing quantitative single-cell data. The approach is particularly powerful when combined with the genetically tractable yeast model organism. The GPCR pathway in yeast is structurally conserved and functionally interchangeable with those in humans. We describe the implementation of a microfluidic device to investigate morphological and transcriptional responses of yeast to a gradient or pulse administration of a GPCR ligand, the peptide mating pheromone alpha-factor"
Keywords:		"Ligands Mating Factor/*metabolism Microfluidics/*instrumentation/*methods Receptors, G-Protein-Coupled/genetics/*metabolism Saccharomyces cerevisiae/genetics/*metabolism Signal Transduction Cell biology G protein-coupled receptor Gradient Microfluidics Ye;"
Notes:		"MedlineSuzuki, Sara Kimiko Kelley, Joshua B Elston, Timothy C Dohlman, Henrik G eng R15 GM128026/GM/NIGMS NIH HHS/ R35 GM118105/GM/NIGMS NIH HHS/ R35 GM127145/GM/NIGMS NIH HHS/ T32 GM067553/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural 2021/06/05 Methods Mol Biol. 2021; 2268:275-287. doi: 10.1007/978-1-0716-1221-7_18"