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Plant Mol Biol

Title:		Chitooligosaccharide elicitor and oxylipins synergistically elevate phytoalexin production in rice
Author(s):		Shinya T; Miyamoto K; Uchida K; Hojo Y; Yumoto E; Okada K; Yamane H; Galis I;
Address:		"Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama, 710-0046, Japan. shinyat@okayama-u.ac.jp. Department of Biosciences, Faculty of Science and Engineering, Teikyo University, Utsunomiya, Tochigi, 320-8551, Japan. Advanced Instrumental Analysis Center, Teikyo University, Utsunomiya, Tochigi, 320-8551, Japan. Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama, 710-0046, Japan. Agro-Biotechnology Research Center, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, 113-8657, Japan"
Journal Title:		Plant Mol Biol
Year:		2022
Volume:		20211125
Issue:		4-May
Page Number:		595 - 609
DOI:		10.1007/s11103-021-01217-w
ISSN/ISBN:		1573-5028 (Electronic) 0167-4412 (Linking)
Abstract:		"We show that in rice, the amino acid-conjugates of JA precursor, OPDA, may function as a non-canonical signal for the production of phytoalexins in coordination with the innate chitin signaling. The core oxylipins, jasmonic acid (JA) and JA-Ile, are well-known as potent regulators of plant defense against necrotrophic pathogens and/or herbivores. However, recent studies also suggest that other oxylipins, including 12-oxo-phytodienoic acid (OPDA), may contribute to plant defense. Here, we used a previously characterized metabolic defense marker, p-coumaroylputrescine (CoP), and fungal elicitor, chitooligosaccharide, to specifically test defense role of various oxylipins in rice (Oryza sativa). While fungal elicitor triggered a rapid production of JA, JA-Ile, and their precursor OPDA, rice cells exogenously treated with the compounds revealed that OPDA, rather than JA-Ile, can stimulate the CoP production. Next, reverse genetic approach and oxylipin-deficient rice mutant (hebiba) were used to uncouple oxylipins from other elicitor-triggered signals. It appeared that, without oxylipins, residual elicitor signaling had only a minimal effect but, in synergy with OPDA, exerted a strong stimulatory activity towards CoP production. Furthermore, as CoP levels were compromised in the OPDA-treated Osjar1 mutant cells impaired in the oxylipin-amino acid conjugation, putative OPDA-amino acid conjugates emerged as hypothetical regulators of CoP biosynthesis. Accordingly, we found several OPDA-amino acid conjugates in rice cells treated with exogenous OPDA, and OPDA-Asp was detected, although in small amounts, in the chitooligosaccharide-treated rice. However, as synthetic OPDA-Asp and OPDA-Ile, so far, failed to induce CoP in cells, it suggests that yet another presumed OPDA-amino acid form(s) could be acting as novel regulator(s) of phytoalexins in rice"
Keywords:		Amino Acids/metabolism Chitin/metabolism Chitosan Cyclopentanes/metabolism Oligosaccharides *Oryza/genetics *Oxylipins/metabolism Sesquiterpenes Phytoalexins Chitin Jasmonic acid Opda Oxylipins Phenolamide Rice;
Notes:		"MedlineShinya, Tomonori Miyamoto, Koji Uchida, Kenichi Hojo, Yuko Yumoto, Emi Okada, Kazunori Yamane, Hisakazu Galis, Ivan eng Grants-in-Aid for Scientific Research/Ministry of Education, Culture, Sports, Science and Technology/ 18K05558/Ministry of Education, Culture, Sports, Science and Technology/ 21K05506/Ministry of Education, Culture, Sports, Science and Technology/ 16K08143/Ministry of Education, Culture, Sports, Science and Technology/ Joint Research Program implemented at the Institute of Plant Science and Resources, Okayama University/Ministry of Education, Culture, Sports, Science and Technology/ Netherlands 2021/11/26 Plant Mol Biol. 2022 Jul; 109(4-5):595-609. doi: 10.1007/s11103-021-01217-w. Epub 2021 Nov 25"