| Title: | A modern purification method for volatile sesquiterpenes produced by recombinant Escherichia coli carrying terpene synthase genes |
| Address: | "a Department of Food and Nutrition , Japan Women's University , Tokyo , Japan" |
| Journal Title: | Biosci Biotechnol Biochem |
| DOI: | 10.1080/09168451.2017.1403882 |
| ISSN/ISBN: | 1347-6947 (Electronic) 0916-8451 (Linking) |
| Abstract: | "Most volatile sesquiterpenes had been purified from plants using distillation and preparative gas chromatography, which is not applicable to many laboratories that do not possess a needed facility. Thus, this review focuses on a modern purification method for volatile sesquiterpenes using Escherichia coli cells that functionally express terpene synthase (Tps) genes. It was recently developed that recombinant E. coli cells carrying Tps genes were cultured in two-layer media (n-octane/TB medium) without harming the cells, and the volatile hydrophobic compounds trapped in the n-octane were purified by two-phase partition (alkane/alkaline 50% MeOH), silica gel column chromatography, and reversed-phase preparative high-performance liquid chromatography (if necessary). Consequently, it was found that the volatile sesquiterpenes are easily purified, the structures of which can then be determined by nuclear magnetic resonance, [alpha](D) and gas chromatography-mass spectrometry analyses. The antioxidant activities of several volatile sesquiterpenes are also presented in this review" |
| Keywords: | "Alkyl and Aryl Transferases/*genetics Antioxidants/pharmacology Chromatography, Liquid/methods Culture Media Escherichia coli/*genetics Gas Chromatography-Mass Spectrometry Magnetic Resonance Spectroscopy *Recombination, Genetic Sesquiterpenes/*isolation;" |
| Notes: | "MedlineShindo, Kazutoshi eng Review England 2017/12/02 Biosci Biotechnol Biochem. 2018 Jun; 82(6):935-939. doi: 10.1080/09168451.2017.1403882. Epub 2017 Dec 1" |
