| Title: | Mutations in pheromone-binding protein3 contribute to pheromone response variations in Plutella xylostella (L.) (Lepidoptera: Plutellidae) |
| Author(s): | Shen J; Hu L; Dai J; Chen B; Zhong G; Zhou X; |
| Address: | "College of Agriculture and Biology, ZhongKai University of Agriculture and Engineering, Guangzhou, China. Guangdong Institute of Applied Biological Resources, Guangzhou, China. Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou, China. College of Chemistry and Chemical Engineering, ZhongKai University of Agriculture and Engineering, Guangzhou, China" |
| ISSN/ISBN: | 1526-4998 (Electronic) 1526-498X (Linking) |
| Abstract: | "BACKGROUND: Diamondback moth Plutella xylostella (L.) (Lepidoptera: Plutellidae) is one of the most important crucifer pests. Commercial sex attractants have been developed to monitor and control P. xylostella. However, some studies have demonstrated a variety of pheromone responses of P. xylostella in different locations of the world. Soluble pheromone-binding proteins (PBPs), as a subfamily of odorant-binding proteins (OBPs), could selectively bind and transport pheromones across aqueous sensillar lymph to the surface of olfactory receptor neurons. It is worthy to study whether the mutation of PxylPBPs is one of the reasons for the different responses of sex attractors in different regions. RESULTS: In this study, P. xylostella males were collected from seven Chinese provinces, including Hainan, Guangdong, Yunnan, Fujian, Hunan, Zhejiang, and Hebei. PxylPBP1, PxylPBP2, and PxylPBP3 were cloned, and 3, 6, and 32 types of mutation pattern were identified, respectively. These mutation patterns were distributed in each province with different frequency. The results of fluorescence displacement binding assay and in silico simulation revealed that the three mutant PxylPBP3 were more sensitive to Z11-16:Ald than the reference protein (ACI28451). CONCLUSION: This result implied that mutation of PxylPBP3 may have contributed to regional differences in pheromone responses of P. xylostella. (c) 2019 Society of Chemical Industry" |
| Keywords: | "Animals China Computer Simulation Geography Ketones/pharmacology Male Molecular Docking Simulation Moths/drug effects/*genetics *Mutation Receptors, Odorant/chemistry/*genetics Sex Attractants/*pharmacology Spectrometry, Fluorescence Plutella xylostella m;" |
| Notes: | "MedlineShen, Jianmei Hu, Liming Dai, Jianqing Chen, Binghan Zhong, Guohua Zhou, Xinhua eng 31401805/National Natural Science Foundation of China/ 2014A020208129/Science and Technology Program of Guangdong Province/ 2015A030313594/Natural Science Foundation of Guangdong Province/ 201607010090/Science and Technology Program of Guangzhou/ KA170500150/Modern Agricultural Production, Teaching and Research Collaborative Education Base 'College Students Innovation Training Program'/ England 2019/01/10 Pest Manag Sci. 2019 Jul; 75(7):2034-2042. doi: 10.1002/ps.5325. Epub 2019 Mar 5" |
