Bedoukian   RussellIPM   RussellIPM   Piezoelectric Micro-Sprayer


Home
Animal Taxa
Plant Taxa
Semiochemicals
Floral Compounds
Semiochemical Detail
Semiochemicals & Taxa
Synthesis
Control
Invasive spp.
References

Abstract

Guide

Alphascents
Pherobio
InsectScience
E-Econex
Counterpart-Semiochemicals
Print
Email to a Friend
Kindly Donate for The Pherobase

« Previous AbstractHematopoietic progenitor kinase 1 (HPK1) negatively regulates prostaglandin E2-induced fos gene transcription    Next AbstractApplication of solid-phase microextraction with gas chromatography and mass spectrometry for the early detection of active moulds on historical woollen objects »

Anal Bioanal Chem


Title:Application of HS-SPME-GC-MS method for the detection of active moulds on historical parchment
Author(s):Sawoszczuk T; Sygula-Cholewinska J; Del Hoyo-Melendez JM;
Address:"Faculty of Commodity Science, Department of Microbiology, Cracow University of Economics, Rakowicka 27, 31-510, Cracow, Poland. tomasz.sawoszczuk@uek.krakow.pl. Faculty of Commodity Science, Department of Microbiology, Cracow University of Economics, Rakowicka 27, 31-510, Cracow, Poland. Laboratory of Analysis and Non-Destructive Investigation of Heritage Objects, National Museum in Krakow, Pilsudskiego 14, 31-106, Cracow, Poland"
Journal Title:Anal Bioanal Chem
Year:2017
Volume:20170111
Issue:9
Page Number:2297 - 2307
DOI: 10.1007/s00216-016-0173-x
ISSN/ISBN:1618-2650 (Electronic) 1618-2642 (Linking)
Abstract:"The goal of this work was to analyse the profile of microbial volatile organic compounds (MVOCs) emitted by moulds growing on parchment samples, in search of particular volatiles mentioned in the literature as indicators of active mould growth. First, the growth of various moulds on samples of parchment was assessed. Those species that showed collagenolytic activity were then inoculated on two types of media: samples of parchment placed on media and on media containing amino acids that are elements of the structure of collagen. All samples were prepared inside 20-ml vials (closed system). In the first case, the media did not contain any sources of organic carbon, nitrogen, or sulphur, i.e. parchment was the only nutrient for the moulds. A third type of sample was historical parchment prepared in a Petri dish without a medium and inoculated with a collagenolytically active mould (open system). The MVOCs emitted by moulds were sampled with the headspace-SPME method. Volatiles extracted on DVB/CAR/PDMS fibres were analysed in a gas chromatography-mass spectrometry system. Qualitative and quantitative analyses of chromatograms were carried out in search of indicators of metabolic activity. The results showed that there are three groups of volatiles that can be used for the detection of active forms of moulds on parchment objects. To the best of our knowledge, this is the first work to measure MVOCs emitted by moulds growing on parchment"
Keywords:Fungi/*isolation & purification Gas Chromatography-Mass Spectrometry/*methods *Paper Solid Phase Microextraction/*methods Biodeterioration Fungi Gc-ms Historical parchment Spme;
Notes:"MedlineSawoszczuk, Tomasz Sygula-Cholewinska, Justyna Del Hoyo-Melendez, Julio M eng Germany 2017/01/13 Anal Bioanal Chem. 2017 Mar; 409(9):2297-2307. doi: 10.1007/s00216-016-0173-x. Epub 2017 Jan 11"

 
Back to top
 
Citation: El-Sayed AM 2024. The Pherobase: Database of Pheromones and Semiochemicals. <http://www.pherobase.com>.
© 2003-2024 The Pherobase - Extensive Database of Pheromones and Semiochemicals. Ashraf M. El-Sayed.
Page created on 27-12-2024