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Sci Rep

Title:		Arabidopsis response to the spider mite Tetranychus urticae depends on the regulation of reactive oxygen species homeostasis
Author(s):		Santamaria ME; Arnaiz A; Velasco-Arroyo B; Grbic V; Diaz I; Martinez M;
Address:		"Centro de Biotecnologia y Genomica de Plantas, Universidad Politecnica de Madrid (UPM) - Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA), Campus Montegancedo UPM, 28223, Pozuelo de Alarcon, Madrid, Spain. Departamento de Biotecnologia-Biologia Vegetal, Escuela Tecnica Superior de Ingenieria Agronomica, Alimentaria y de Biosistemas, UPM, 28040, Madrid, Spain. Department of Biology, The University of Western Ontario, 1151 Richmond St, London, ON, N6A 5B7, Canada. Centro de Biotecnologia y Genomica de Plantas, Universidad Politecnica de Madrid (UPM) - Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA), Campus Montegancedo UPM, 28223, Pozuelo de Alarcon, Madrid, Spain. m.martinez@upm.es. Departamento de Biotecnologia-Biologia Vegetal, Escuela Tecnica Superior de Ingenieria Agronomica, Alimentaria y de Biosistemas, UPM, 28040, Madrid, Spain. m.martinez@upm.es"
Journal Title:		Sci Rep
Year:		2018
Volume:		20180621
Issue:		1
Page Number:		9432 -
DOI:		10.1038/s41598-018-27904-1
ISSN/ISBN:		2045-2322 (Electronic) 2045-2322 (Linking)
Abstract:		"Reactive oxygen species (ROS) are molecules that play a prominent role in plant response to numerous stresses, including plant interactions with herbivores. Previous findings indicate that Arabidopsis plants showed an increase in H(2)O(2) accumulation after Tetranychus urticae infestation. Despite its importance, no information has been reported on the relationships between ROS-metabolizing systems and the spider mite-triggered plant-induced responses. In this work, four ROS-related genes that were differentially expressed between the resistant Bla-2 and the susceptible Kon Arabidopsis accessions were selected for the analysis. These genes encode proteins putatively involved in the generation (BBE22) and degradation (GPX7 and GSTU4) of H(2)O(2), and in the degradation of ascorbate (AO). Overexpressing BBE22 and silencing GPX7, GSTU4 and AO resulted in higher leaf damage and better mite performance relative to the wild-type plants. Minor effects on H(2)O(2) accumulation obscure major effects on the expression of genes related to ROS-metabolism and JA and SA signaling pathways, and on ROS-related enzymatic activities. In conclusion, the integration of ROS and ROS-related compounds and enzymes in the response of Arabidopsis to the spider mite T. urticae was confirmed. However, the complex network involved in ROS signaling makes difficult to predict the impact of a specific genetic manipulation"
Keywords:		Animals Arabidopsis/genetics/*metabolism/parasitology Arabidopsis Proteins/genetics/metabolism Glutathione Transferase/genetics/metabolism *Homeostasis *Host-Parasite Interactions Hydrogen Peroxide/*metabolism Peroxidases/genetics/metabolism Tetranychidae;
Notes:		"MedlineSantamaria, M Estrella Arnaiz, Ana Velasco-Arroyo, Blanca Grbic, Vojislava Diaz, Isabel Martinez, Manuel eng Research Support, Non-U.S. Gov't England 2018/06/23 Sci Rep. 2018 Jun 21; 8(1):9432. doi: 10.1038/s41598-018-27904-1"