« Previous AbstractAlternative Ecosorbent for the Determination of Trihalomethanes in Aqueous Samples in SPME Mode    Next AbstractEvolution of volatile compounds and sensory characteristics of edible green seaweed (Ulva rigida) during storage at different temperatures »

Microb Ecol

Title:		Antifungal Volatile Organic Compounds from the Endophyte Nodulisporium sp. Strain GS4d2II1a: a Qualitative Change in the Intraspecific and Interspecific Interactions with Pythium aphanidermatum
Author(s):		Sanchez-Fernandez RE; Diaz D; Duarte G; Lappe-Oliveras P; Sanchez S; Macias-Rubalcava ML;
Address:		"Instituto de Quimica, Departamento de Productos Naturales, Universidad Nacional Autonoma de Mexico (UNAM). Ciudad Universitaria, Coyoacan, D.F., 04510, Mexico. Instituto de Investigaciones Biomedicas, Departamento de Biologia Celular y Fisiologia, UNAM. Ciudad Universitaria, Coyoacan, D.F., 04510, Mexico. Facultad de Quimica, Unidad de Servicios de Apoyo a la Investigacion, UNAM. Ciudad Universitaria, Coyoacan, D.F., 04510, Mexico. Instituto de Biologia, Departamento de Botanica, UNAM. Ciudad Universitaria, Coyoacan, D.F., 04510, Mexico. Instituto de Investigaciones Biomedicas, Departamento de Biologia Molecular y Biotecnologia, UNAM. Ciudad Universitaria, Coyoacan, D.F., 04510, Mexico. Instituto de Quimica, Departamento de Productos Naturales, Universidad Nacional Autonoma de Mexico (UNAM). Ciudad Universitaria, Coyoacan, D.F., 04510, Mexico. mmacias@iquimica.unam.mx"
Journal Title:		Microb Ecol
Year:		2016
Volume:		20150926
Issue:		2
Page Number:		347 - 364
DOI:		10.1007/s00248-015-0679-3
ISSN/ISBN:		1432-184X (Electronic) 0095-3628 (Linking)
Abstract:		"This study demonstrates volatile organic compounds (VOCs) production as one of the defense mechanisms of the antagonistic endophyte Nodulisporium sp. GS4d2II1a, and the volatile changes in two times of the fungal growth; and, as result of its intra and interspecific interactions with the plant pathogen Pythium aphanidermatum. The antifungal activity of the volatile and diffusible metabolites was evaluated by means of three types of antagonism bioassays and by organic extract agar dilution. VOCs were obtained by gas chromatography coupled to mass spectrometry from 3- and 5-day Nodulisporium sp. cultures, as well as from its interspecific in vitro antagonistic interaction with the oomycete P. aphanidermatum, and its intraspecific Nodulisporium sp.-Nodulisporium sp. interaction. The GS4d2II1a strain completely inhibited the growth of two fungi and seven oomycetes by replacing their mycelia in simple antagonism bioassays and by producing in vitro volatile and diffusible metabolites that acted synergistically in multiple antagonism bioassays. Additionally, VOCs inhibited the growth of three oomycetes and one fungus in antagonism bioassays using divided plates. A total of 70 VOCs were detected, mainly including mono and sesquiterpenes, especially eucalyptol and limonene. Multiple correspondence analysis revealed four different volatile profiles, showing that volatiles changed with the fungus age and its intra and interspecific interactions. The metabolites produced by Nodulisporium sp. GS4d2II1a could be useful for biological control of fungal and oomycetes plant pathogens of economically important crops"
Keywords:		Antifungal Agents/chemistry/metabolism/*pharmacology Endophytes/*chemistry/metabolism Plant Diseases/*microbiology Pythium/*drug effects/growth & development/physiology Volatile Organic Compounds/chemistry/metabolism/*pharmacology Xylariales/*chemistry/gr;
Notes:		"MedlineSanchez-Fernandez, Rosa Elvira Diaz, Daniel Duarte, Georgina Lappe-Oliveras, Patricia Sanchez, Sergio Macias-Rubalcava, Martha Lydia eng Research Support, Non-U.S. Gov't 2015/09/27 Microb Ecol. 2016 Feb; 71(2):347-64. doi: 10.1007/s00248-015-0679-3. Epub 2015 Sep 26"