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Insect Biochem Mol Biol

Title:		"Cloning and functional characterization of a fatty acid transport protein (FATP) from the pheromone gland of a lichen moth, Eilema japonica, which secretes an alkenyl sex pheromone"
Author(s):		Qian S; Fujii T; Ito K; Nakano R; Ishikawa Y;
Address:		"Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan"
Journal Title:		Insect Biochem Mol Biol
Year:		2011
Volume:		20100927
Issue:		1
Page Number:		22 - 28
DOI:		10.1016/j.ibmb.2010.09.008
ISSN/ISBN:		1879-0240 (Electronic) 0965-1748 (Linking)
Abstract:		"Sex pheromones of moths are largely classified into two types based on the presence (Type I) or absence (Type II) of a terminal functional group. While Type-I sex pheromones are synthesized from common fatty acids in the pheromone gland (PG), Type-II sex pheromones are derived from hydrocarbons produced presumably in the oenocytes and transported to the PG via the hemolymph. Recently, a fatty acid transport protein (BmFATP) was identified from the PG of the silkworm Bombyx mori, which produces a Type-I sex pheromone (bombykol). BmFATP was shown to facilitate the uptake of extracellular fatty acids into PG cells for the synthesis of bombykol. To elucidate the presence and function of FATP in the PG of moths that produce Type-II sex pheromones, we explored fatp homologues expressed in the PG of a lichen moth, Eilema japonica, which secretes an alkenyl sex pheromone (Type II). A fatp homologue cloned from E. japonica (Ejfatp) was predominantly expressed in the PG, and its expression is upregulated shortly after eclosion. Functional expression of EjFATP in Escherichia coli enhanced the uptake of long chain fatty acids (C(1)(8) and C(2)(0)), but not pheromone precursor hydrocarbons. To the best of our knowledge, this is the first report of the cloning and functional characterization of a FATP in the PG of a moth producing a Type-II sex pheromone. Although EjFATP is not likely to be involved in the uptake of pheromone precursors in E. japonica, the expression pattern of Ejfatp suggests a role for EjFATP in the PG not directly linked to pheromone biosynthesis"
Keywords:		"Amino Acid Sequence Animals Biological Transport/genetics Cloning, Molecular Escherichia coli Fatty Acid Transport Proteins/*genetics/isolation & purification/metabolism Fatty Acids/metabolism Female Hemolymph/metabolism Insect Proteins/*genetics/isolatio;"
Notes:		"MedlineQian, Shuguang Fujii, Takeshi Ito, Katsuhiko Nakano, Ryo Ishikawa, Yukio eng Research Support, Non-U.S. Gov't England 2010/09/30 Insect Biochem Mol Biol. 2011 Jan; 41(1):22-8. doi: 10.1016/j.ibmb.2010.09.008. Epub 2010 Sep 27"