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J Chem Ecol

Title:		Jasmonic Acid and Ethylene Signaling Pathways Regulate Glucosinolate Levels in Plants During Rhizobacteria-Induced Systemic Resistance Against a Leaf-Chewing Herbivore
Author(s):		Pangesti N; Reichelt M; van de Mortel JE; Kapsomenou E; Gershenzon J; van Loon JJ; Dicke M; Pineda A;
Address:		"Laboratory of Entomology, Wageningen University, P.O. Box 16, 6700 AA, Wageningen, The Netherlands. npangesti001@gmail.com. Department of Biochemistry, Max Planck Institute for Chemical Ecology, 07745, Jena, Germany. Laboratory of Phytopathology, Wageningen University, P.O. Box 16, 6700 AA, Wageningen, The Netherlands. HAS University of Applied Sciences, 5911 KJ, Venlo, The Netherlands. Laboratory of Entomology, Wageningen University, P.O. Box 16, 6700 AA, Wageningen, The Netherlands. Department of Terrestrial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), PO Box 50, 6700 AB, Wageningen, The Netherlands"
Journal Title:		J Chem Ecol
Year:		2016
Volume:		20161115
Issue:		12
Page Number:		1212 - 1225
DOI:		10.1007/s10886-016-0787-7
ISSN/ISBN:		1573-1561 (Electronic) 0098-0331 (Print) 0098-0331 (Linking)
Abstract:		"Beneficial soil microbes can promote plant growth and induce systemic resistance (ISR) in aboveground tissues against pathogens and herbivorous insects. Despite the increasing interest in microbial-ISR against herbivores, the underlying molecular and chemical mechanisms of this phenomenon remain elusive. Using Arabidopsis thaliana and the rhizobacterium Pseudomonas simiae WCS417r (formerly known as P. fluorescens WCS417r), we here evaluate the role of the JA-regulated MYC2-branch and the JA/ET-regulated ORA59-branch in modulating rhizobacteria-ISR to Mamestra brassicae by combining gene transcriptional, phytochemical, and herbivore performance assays. Our data show a consistent negative effect of rhizobacteria-mediated ISR on the performance of M. brassicae. Functional JA- and ET-signaling pathways are required for this effect, as shown by investigating the knock-out mutants dde2-2 and ein2-1. Additionally, whereas herbivory mainly induces the MYC2-branch, rhizobacterial colonization alone or in combination with herbivore infestation induces the ORA59-branch of the JA signaling pathway. Rhizobacterial colonization enhances the synthesis of camalexin and aliphatic glucosinolates (GLS) compared to the control, while it suppresses the herbivore-induced levels of indole GLS. These changes are associated with modulation of the JA-/ET-signaling pathways. Our data show that the colonization of plant roots by rhizobacteria modulates plant-insect interactions by prioritizing the JA/ET-regulated ORA59-branch over the JA-regulated MYC2-branch. This study elucidates how microbial plant symbionts can modulate the plant immune system to mount an effective defense response against herbivorous plant attackers"
Keywords:		"Animals Arabidopsis/genetics/microbiology/*physiology Arabidopsis Proteins/genetics/metabolism Basic Helix-Loop-Helix Leucine Zipper Transcription Factors/genetics/metabolism Cyclopentanes/*metabolism Ethylenes/*metabolism Gene Expression Regulation, Plan;"
Notes:		"MedlinePangesti, Nurmi Reichelt, Michael van de Mortel, Judith E Kapsomenou, Eleni Gershenzon, Jonathan van Loon, Joop J A Dicke, Marcel Pineda, Ana eng 2016/11/17 J Chem Ecol. 2016 Dec; 42(12):1212-1225. doi: 10.1007/s10886-016-0787-7. Epub 2016 Nov 15"