« Previous AbstractInhibition of virulence factor expression in Staphylococcus aureus by the Staphylococcus epidermidis agr pheromone and derivatives    Next AbstractCultured and uncultured microbial community associated with biogas production in anaerobic digestion processes »

Toxins (Basel)

Title:		Combining E-Nose and Lateral Flow Immunoassays (LFIAs) for Rapid Occurrence/Co-Occurrence Aflatoxin and Fumonisin Detection in Maize
Author(s):		Ottoboni M; Pinotti L; Tretola M; Giromini C; Fusi E; Rebucci R; Grillo M; Tassoni L; Foresta S; Gastaldello S; Furlan V; Maran C; Dell'Orto V; Cheli F;
Address:		"Department of Health, Animal Science and Food Safety, Universita degli Studi di Milano, Via Trentacoste, 2, 20134 Milan, Italy. matteo.ottoboni@unimi.it. Department of Health, Animal Science and Food Safety, Universita degli Studi di Milano, Via Trentacoste, 2, 20134 Milan, Italy. luciano.pinotti@unimi.it. Department of Health, Animal Science and Food Safety, Universita degli Studi di Milano, Via Trentacoste, 2, 20134 Milan, Italy. marco.tretola@unimi.it. Department of Health, Animal Science and Food Safety, Universita degli Studi di Milano, Via Trentacoste, 2, 20134 Milan, Italy. carlotta.giromini@unimi.it. Department of Health, Animal Science and Food Safety, Universita degli Studi di Milano, Via Trentacoste, 2, 20134 Milan, Italy. eleonora.fusi@unimi.it. Department of Health, Animal Science and Food Safety, Universita degli Studi di Milano, Via Trentacoste, 2, 20134 Milan, Italy. raffaella.rebucci@unimi.it. ATPr&d S.r.l., Via Ca' Marzare, 3, Camisano Vicentino, 36043 Vicenza, Italy. mariagrillo@atprd.it. ATPr&d S.r.l., Via Ca' Marzare, 3, Camisano Vicentino, 36043 Vicenza, Italy. lucatassoni@atprd.it. ATPr&d S.r.l., Via Ca' Marzare, 3, Camisano Vicentino, 36043 Vicenza, Italy. silvia.foresta@live.it. ATPr&d S.r.l., Via Ca' Marzare, 3, Camisano Vicentino, 36043 Vicenza, Italy. silviagastaldello@atprd.it. CerealDocks S.p.A., Via dell'Agricoltura, 10, Summaga di Portogruaro, 30026 Venezia, Italy. valentinafurlan@cerealdocks.it. ATPr&d S.r.l., Via Ca' Marzare, 3, Camisano Vicentino, 36043 Vicenza, Italy. claudiomaran@atprd.it. Department of Health, Animal Science and Food Safety, Universita degli Studi di Milano, Via Trentacoste, 2, 20134 Milan, Italy. vittorio.dellorto@unimi.it. Department of Health, Animal Science and Food Safety, Universita degli Studi di Milano, Via Trentacoste, 2, 20134 Milan, Italy. federica.cheli@unimi.it"
Journal Title:		Toxins (Basel)
Year:		2018
Volume:		20181016
Issue:		10
Page Number:		-
DOI:		10.3390/toxins10100416
ISSN/ISBN:		2072-6651 (Electronic) 2072-6651 (Linking)
Abstract:		"The aim of this study was to evaluate the potential use of an e-nose in combination with lateral flow immunoassays for rapid aflatoxin and fumonisin occurrence/co-occurrence detection in maize samples. For this purpose, 161 samples of corn have been used. Below the regulatory limits, single-contaminated, and co-contaminated samples were classified according to the detection ranges established for commercial lateral flow immunoassays (LFIAs) for mycotoxin determination. Correspondence between methods was evaluated by discriminant function analysis (DFA) procedures using IBM SPSS Statistics 22. Stepwise variable selection was done to select the e-nose sensors for classifying samples by DFA. The overall leave-out-one cross-validated percentage of samples correctly classified by the eight-variate DFA model for aflatoxin was 81%. The overall leave-out-one cross-validated percentage of samples correctly classified by the seven-variate DFA model for fumonisin was 85%. The overall leave-out-one cross-validated percentage of samples correctly classified by the nine-variate DFA model for the three classes of contamination (below the regulatory limits, single-contaminated, co-contaminated) was 65%. Therefore, even though an exhaustive evaluation will require a larger dataset to perform a validation procedure, an electronic nose (e-nose) seems to be a promising rapid/screening method to detect contamination by aflatoxin, fumonisin, or both in maize kernel stocks"
Keywords:		"Aflatoxins/*analysis/immunology Antibodies, Immobilized/immunology Electronic Nose Food Contamination/*analysis Fumonisins/*analysis/immunology Immunoassay *Zea mays Zea mays aflatoxin co-contamination discriminant analysis fumonisin volatile organic comp;"
Notes:		"MedlineOttoboni, Matteo Pinotti, Luciano Tretola, Marco Giromini, Carlotta Fusi, Eleonora Rebucci, Raffaella Grillo, Maria Tassoni, Luca Foresta, Silvia Gastaldello, Silvia Furlan, Valentina Maran, Claudio Dell'Orto, Vittorio Cheli, Federica eng Research Support, Non-U.S. Gov't Switzerland 2018/10/20 Toxins (Basel). 2018 Oct 16; 10(10):416. doi: 10.3390/toxins10100416"