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Pathogens

Title:		"Fusaricidins, Polymyxins and Volatiles Produced by Paenibacillus polymyxa Strains DSM 32871 and M1"
Author(s):		Mulner P; Schwarz E; Dietel K; Herfort S; Jahne J; Lasch P; Cernava T; Berg G; Vater J;
Address:		"ABITEP GmbH, Glienicker Weg 185, 12489 Berlin, Germany. Institute of Environmental Biotechnology, Graz University of Technology, Petergasse 12, 8010 Graz, Austria. Robert Koch-Institut, ZBS6, Proteomics and Spectroscopy, Seestr 10, 13353 Berlin, Germany"
Journal Title:		Pathogens
Year:		2021
Volume:		20211115
Issue:		11
Page Number:		-
DOI:		10.3390/pathogens10111485
ISSN/ISBN:		2076-0817 (Print) 2076-0817 (Electronic) 2076-0817 (Linking)
Abstract:		"Paenibacilli are efficient producers of potent agents against bacterial and fungal pathogens, which are of great interest both for therapeutic applications in medicine as well as in agrobiotechnology. Lipopeptides produced by such organisms play a major role in their potential to inactivate pathogens. In this work we investigated two lipopeptide complexes, the fusaricidins and the polymyxins, produced by Paenibacillus polymyxa strains DSM 32871 and M1 by MALDI-TOF mass spectrometry. The fusaricidins show potent antifungal activities and are distinguished by an unusual variability. For strain DSM 32871 we identified numerous yet unknown variants mass spectrometrically. DSM 32871 produces polymyxins of type E (colistins), while M1 forms polymyxins P. For both strains, novel but not yet completely characterized polymyxin species were detected, which possibly are glycosylated. These compounds may be of interest therapeutically, because polymyxins have gained increasing attention as last-resort antibiotics against multiresistant pathogenic Gram-negative bacteria. In addition, the volatilomes of DSM 32781 and M1 were investigated with a GC-MS approach using different cultivation media. Production of volatile organic compounds (VOCs) was strain and medium dependent. In particular, strain M1 manifested as an efficient VOC-producer that exhibited formation of 25 volatiles in total. A characteristic feature of Paenibacilli is the formation of volatile pyrazine derivatives"
Keywords:		Gc-ms MALDI-TOF mass spectrometry P.polymyxa bioactive peptides volatiles;
Notes:		"PubMed-not-MEDLINEMulner, Pascal Schwarz, Elisa Dietel, Kristin Herfort, Stefanie Jahne, Jennifer Lasch, Peter Cernava, Tomislav Berg, Gabriele Vater, Joachim eng Switzerland 2021/11/28 Pathogens. 2021 Nov 15; 10(11):1485. doi: 10.3390/pathogens10111485"