| Title: | Cooperative binding interactions required for function of the Ty1 sterile responsive element |
| Author(s): | Baur M; Esch RK; Errede B; |
| Address: | "Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill 27599-7260, USA" |
| ISSN/ISBN: | 0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking) |
| Abstract: | "The Ste12p transcription factor controls the expression of Ty1 transposable element insertion mutations and genes whose products are required for mating in Saccharomyces cerevisiae. The binding site for Ste12p is a consensus DNA sequence known as a pheromone response element (PRE). Upstream activating sequences (UASs) derived from known Ste12p-dependent genes have previously been characterized to require either multiple PREs or a single PRE coupled to a binding site for a second protein. The Ste12p-dependent UAS from Ty1, called a sterile response element (SRE), is of the second type and is comprised of a PRE and an adjacent TEA (TEF-1, Tec1, and AbaA motif) DNA consensus sequence (TCS). In this report, we show by UV cross-linking analysis that two proteins, Ste12p and a protein with an apparent size of 72 kDa, directly contact the Ty1 SRE. Other experiments show that Tec1p is required for formation of the Ty1 SRE protein-DNA complex and is physically present in the complex. These results establish a direct role for Tec1p in the Ty1 SRE and yet another set of combinatorial interactions that achieve a qualitatively distinct mode of transcriptional regulation with Ste12p" |
| Keywords: | "Base Sequence Consensus Sequence Cross-Linking Reagents DNA Transposable Elements/*physiology DNA, Fungal/*metabolism DNA-Binding Proteins/chemistry/*metabolism Fungal Proteins/*metabolism Gene Expression Regulation, Fungal/genetics Molecular Sequence Dat;" |
| Notes: | "MedlineBaur, M Esch, R K Errede, B eng GM-30619/GM/NIGMS NIH HHS/ GM-39852/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, P.H.S. 1997/08/01 Mol Cell Biol. 1997 Aug; 17(8):4330-7. doi: 10.1128/MCB.17.8.4330" |
