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Nat Commun

Title:		Microbial volatile communication in human organotypic lung models
Author(s):		Barkal LJ; Procknow CL; Alvarez-Garcia YR; Niu M; Jimenez-Torres JA; Brockman-Schneider RA; Gern JE; Denlinger LC; Theberge AB; Keller NP; Berthier E; Beebe DJ;
Address:		"Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, 53706, USA. Carbone Cancer Center, University of Wisconsin-Madison, Madison, WI, 53705, USA. Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, 53706, USA. Department of Chemistry, University of Wisconsin-Madison, Madison, WI, 53706, USA. Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI, 53706, USA. Department of Pediatrics, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, 53705, USA. Department of Medicine, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, 53705, USA. Department of Chemistry, University of Washington, Seattle, WA, 98195, USA. Department of Urology, University of Washington School of Medicine, Seattle, WA, 98195, USA. Department of Chemistry, University of Washington, Seattle, WA, 98195, USA. erwin.berthier@gmail.com. Tasso Inc., Seattle, WA, 98119, USA. erwin.berthier@gmail.com. Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, 53706, USA. djbeebe@wisc.edu. Carbone Cancer Center, University of Wisconsin-Madison, Madison, WI, 53705, USA. djbeebe@wisc.edu"
Journal Title:		Nat Commun
Year:		2017
Volume:		20171124
Issue:		1
Page Number:		1770 -
DOI:		10.1038/s41467-017-01985-4
ISSN/ISBN:		2041-1723 (Electronic) 2041-1723 (Linking)
Abstract:		"We inhale respiratory pathogens continuously, and the subsequent signaling events between host and microbe are complex, ultimately resulting in clearance of the microbe, stable colonization of the host, or active disease. Traditional in vitro methods are ill-equipped to study these critical events in the context of the lung microenvironment. Here we introduce a microscale organotypic model of the human bronchiole for studying pulmonary infection. By leveraging microscale techniques, the model is designed to approximate the structure of the human bronchiole, containing airway, vascular, and extracellular matrix compartments. To complement direct infection of the organotypic bronchiole, we present a clickable extension that facilitates volatile compound communication between microbial populations and the host model. Using Aspergillus fumigatus, a respiratory pathogen, we characterize the inflammatory response of the organotypic bronchiole to infection. Finally, we demonstrate multikingdom, volatile-mediated communication between the organotypic bronchiole and cultures of Aspergillus fumigatus and Pseudomonas aeruginosa"
Keywords:		"Aspergillosis/immunology/microbiology Aspergillus fumigatus/chemistry/*metabolism Bronchioles/immunology/*microbiology Cytokines/immunology Host-Pathogen Interactions Humans Lung Diseases/microbiology Models, Biological Pseudomonas Infections/immunology/m;"
Notes:		"MedlineBarkal, Layla J Procknow, Clare L Alvarez-Garcia, Yasmin R Niu, Mengyao Jimenez-Torres, Jose A Brockman-Schneider, Rebecca A Gern, James E Denlinger, Loren C Theberge, Ashleigh B Keller, Nancy P Berthier, Erwin Beebe, David J eng R01 HL115118/HL/NHLBI NIH HHS/ K12 DK100022/DK/NIDDK NIH HHS/ P30 CA014520/CA/NCI NIH HHS/ T32 GM008692/GM/NIGMS NIH HHS/ T15 LM007359/LM/NLM NIH HHS/ R01 AI065728/AI/NIAID NIH HHS/ R01 CA155192/CA/NCI NIH HHS/ U19 AI104317/AI/NIAID NIH HHS/ T32 HL007899/HL/NHLBI NIH HHS/ P01 HL070831/HL/NHLBI NIH HHS/ R01 EB010039/EB/NIBIB NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2017/11/28 Nat Commun. 2017 Nov 24; 8(1):1770. doi: 10.1038/s41467-017-01985-4"