| Title: | The gelatinase biosynthesis-activating pheromone binds and stabilises the FsrB membrane protein in Enterococcus faecalis quorum sensing |
| Author(s): | Littlewood S; Tattersall H; Hughes CS; Hussain R; Ma P; Harding SE; Nakayama J; Phillips-Jones MK; |
| Address: | "School of Pharmacy and Biomedical Sciences, University of Central Lancashire, Preston, UK. Diamond Light Source Ltd., Harwell Science and Innovation Campus, Didcot, UK. Astbury Centre for Structural Molecular Biology, University of Leeds, UK. National Centre for Macromolecular Hydrodynamics, School of Biosciences, University of Nottingham, Sutton Bonington, UK. Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan" |
| ISSN/ISBN: | 1873-3468 (Electronic) 0014-5793 (Print) 0014-5793 (Linking) |
| Abstract: | "Quorum-sensing mechanisms regulate gene expression in response to changing cell-population density detected through pheromones. In Enterococcus faecalis, Fsr quorum sensing produces and responds to the gelatinase biosynthesis-activating pheromone (GBAP). Here we establish that the enterococcal FsrB membrane protein has a direct role connected with GBAP by showing that GBAP binds to purified FsrB. Far-UV CD measurements demonstrated a predominantly alpha-helical protein exhibiting a small level of conformational flexibility. Fivefold (400 mum) GBAP stabilised FsrB (80 mum) secondary structure. FsrB thermal denaturation in the presence and absence of GBAP revealed melting temperatures of 70.1 and 60.8 degrees C, respectively, demonstrating GBAP interactions and increased thermal stability conferred by GBAP. Addition of GBAP also resulted in tertiary structural changes, confirming GBAP binding" |
| Keywords: | "Amino Acid Sequence Bacterial Proteins/chemistry/*metabolism Enterococcus faecalis/*cytology/drug effects/*metabolism Lactones/*metabolism/*pharmacology Peptides, Cyclic/*metabolism/*pharmacology Protein Binding Protein Conformation, alpha-Helical Protein;" |
| Notes: | "MedlineLittlewood, Sean Tattersall, Helena Hughes, Charlotte S Hussain, Rohanah Ma, Pikyee Harding, Stephen E Nakayama, Jiro Phillips-Jones, Mary K eng BB/D001641/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom Research Support, Non-U.S. Gov't England 2019/10/11 FEBS Lett. 2020 Feb; 594(3):553-563. doi: 10.1002/1873-3468.13634. Epub 2019 Oct 21" |
