| Title: | (13) C-chloromethane incubations provide evidence for novel bacterial chloromethane degraders in a living tree fern |
| Author(s): | Krober E; Wende S; Kanukollu S; Buchen-Tschiskale C; Besaury L; Keppler F; Vuilleumier S; Kolb S; Bringel F; |
| Address: | "Microbial Biogeochemistry, RA Landscape Functioning, ZALF Leibniz Centre for Agricultural Landscape Research, Muncheberg, Germany. Isotope Biogeochemistry and Gas Fluxes, RA Landscape Functioning, ZALF Leibniz Centre for Agricultural Landscape Research, Muncheberg, Germany. Genetique Moleculaire, Genomique, Microbiologie (GMGM), Universite de Strasbourg, UMR 7156 CNRS, Strasbourg, France. Institute of Earth Sciences, Heidelberg University, Heidelberg, Germany. Thaer Institute, Faculty of Life Sciences, Humboldt University of Berlin, Berlin, Germany" |
| ISSN/ISBN: | 1462-2920 (Electronic) 1462-2912 (Linking) |
| Abstract: | "Chloromethane (CH(3) Cl) is the most abundant halogenated volatile organic compound in the atmosphere and contributes to stratospheric ozone depletion. CH(3) Cl has mainly natural sources such as emissions from vegetation. In particular, ferns have been recognized as strong emitters. Mitigation of CH(3) Cl to the atmosphere by methylotrophic bacteria, a global sink for this compound, is likely underestimated and remains poorly characterized. We identified and characterized CH(3) Cl-degrading bacteria associated with intact and living tree fern plants of the species Cyathea australis by stable isotope probing (SIP) with (13) C-labelled CH(3) Cl combined with metagenomics. Metagenome-assembled genomes (MAGs) related to Methylobacterium and Friedmanniella were identified as being involved in the degradation of CH(3) Cl in the phyllosphere, i.e., the aerial parts of the tree fern, while a MAG related to Sorangium was linked to CH(3) Cl degradation in the fern rhizosphere. The only known metabolic pathway for CH(3) Cl degradation, via a methyltransferase system including the gene cmuA, was not detected in metagenomes or MAGs identified by SIP. Hence, a yet uncharacterized methylotrophic cmuA-independent pathway may drive CH(3) Cl degradation in the investigated tree ferns" |
| Keywords: | Atmosphere Bacteria/genetics *Ferns *Methyl Chloride Methyltransferases; |
| Notes: | "MedlineKrober, Eileen Wende, Sonja Kanukollu, Saranya Buchen-Tschiskale, Caroline Besaury, Ludovic Keppler, Frank Vuilleumier, Stephane Kolb, Steffen Bringel, Francoise eng Research Support, Non-U.S. Gov't England 2021/06/15 Environ Microbiol. 2021 Aug; 23(8):4450-4465. doi: 10.1111/1462-2920.15638. Epub 2021 Jun 27" |
