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Eur J Neurosci

Title:		Bicuculline induces synapse formation on primary cultured accessory olfactory bulb neurons
Author(s):		Kato-Negishi M; Muramoto K; Kawahara M; Hosoda R; Kuroda Y; Ichikawa M;
Address:		"Laboratory of Anatomy and Cell Biology, Department of Basic Technique and Facilities, Tokyo Metropolitan Institute for Neuroscience, Tokyo Metropolitan Organization for Medical Research, 2-6 Musashi-dai Fuchu, Tokyo 183-8526, Japan. midori@tmin.ac.jp"
Journal Title:		Eur J Neurosci
Year:		2003
Volume:		18
Issue:		6
Page Number:		1343 - 1352
DOI:		10.1046/j.1460-9568.2003.02901.x
ISSN/ISBN:		0953-816X (Print) 0953-816X (Linking)
Abstract:		"To investigate the roles of the GABAergic inhibitory system of accessory olfactory bulb (AOB) in pheromonal memory formation, we have developed a primary culture system of AOB neurons, which had numerous excitatory and inhibitory synapses. Using this culture system of AOB neurons, we examined the correlation in rats between neuronal excitation and synaptic morphology by bicuculline-induced disinhibition of cultured AOB neurons. The exposure to bicuculline induced long-lasting oscillatory changes in the intracellular calcium level ([Ca2+]in) of cultured non-GABAergic multipolar neurons, which were identified as mitral/tufted cells (MT cells). These MT cells exhibited the appearance of dendritic filopodia structures after a 10-min treatment with bicuculline. By labelling presynaptic terminals with FM4-64, the appearance of new presynaptic terminals was clearly observed on newly formed filopodia after 120 min treatment with bicuculline. These results suggest that bicuculline-induced [Ca2+]in oscillation of MT cells induces the growth of filopodia and subsequently the formation of new presynaptic terminals. Furthermore, tetrodotoxin or the deprivation of extracellular calcium blocked bicuculline-induced synapse formation. The present results indicate that the long-lasting [Ca2+]in oscillation caused by bicuculline-induced disinhibition of cultured MT cells is significantly implicated in the mechanism underlying synapse formation on cultured AOB neurons. Our established culture system of AOB neurons will aid in clarifying the mechanism of synapse formation between AOB neurons and the molecular mechanism of pheromonal memory formation"
Keywords:		"Actins/metabolism Anesthetics, Local/pharmacology Animals Animals, Newborn Bicuculline/*pharmacology Calcium/metabolism Calcium Signaling Cells, Cultured Dendrites/drug effects/metabolism Fura-2/metabolism GABA Antagonists/*pharmacology Glutamate Decarbox;"
Notes:		"MedlineKato-Negishi, Midori Muramoto, Kazuyo Kawahara, Masahiro Hosoda, Ritsuko Kuroda, Yoichiro Ichikawa, Masumi eng Comparative Study Research Support, Non-U.S. Gov't France 2003/09/27 Eur J Neurosci. 2003 Sep; 18(6):1343-52. doi: 10.1046/j.1460-9568.2003.02901.x"