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Environ Sci Technol

Title:		Characterization of Adipogenic Activity of House Dust Extracts and Semi-Volatile Indoor Contaminants in 3T3-L1 Cells
Author(s):		Kassotis CD; Hoffman K; Stapleton HM;
Address:		"Nicholas School of the Environment, Duke University , Durham, North Carolina 27708, United States"
Journal Title:		Environ Sci Technol
Year:		2017
Volume:		20170712
Issue:		15
Page Number:		8735 - 8745
DOI:		10.1021/acs.est.7b01788
ISSN/ISBN:		1520-5851 (Electronic) 0013-936X (Print) 0013-936X (Linking)
Abstract:		"Obesity and metabolic disorders are of great societal concern and generate significant human health care costs. Recently, attention has focused on the potential for environmental contaminants to act as metabolic disruptors. This study sought to evaluate the adipogenic activity of indoor house dust extracts and a suite of semivolatile organic chemicals (SVOCs) that are often ubiquitously detected in indoor environments. 3T3-L1 cells were exposed to extracts of indoor dust or individual SVOCs and assessed for triglyceride accumulation and preadipocyte proliferation. Ten of 11 house dust extracts exhibited significant triglyceride accumulation and/or proliferation at environmentally relevant levels (<20 mug of dust/well), and significant adipogenic activity was also exhibited by 28 of the SVOCs. Notably, pyraclostrobin, dibutyl phthalate, tert-butyl-phenyl diphenyl phosphate, and the isopropylated triaryl phosphates (ITPs) exhibited near maximal or supra-maximal triglyceride accumulation relative to the rosiglitazone-induced maximum. The adipogenic activity in house dust occurred at concentrations below EPA estimated child exposure levels, and raises concerns for human health impacts, particularly in children. Our results delineate a novel potential health threat and identify putative causative SVOCs that are likely contributing to this activity"
Keywords:		"3T3-L1 Cells Air Pollutants/*toxicity Air Pollution, Indoor Animals Child Dibutyl Phthalate *Dust Humans Mice Risk Assessment Volatile Organic Compounds;"
Notes:		"MedlineKassotis, Christopher D Hoffman, Kate Stapleton, Heather M eng P42 ES010356/ES/NIEHS NIH HHS/ R01 ES016099/ES/NIEHS NIH HHS/ 2017/07/13 Environ Sci Technol. 2017 Aug 1; 51(15):8735-8745. doi: 10.1021/acs.est.7b01788. Epub 2017 Jul 12"