| Title: | Identification and characterisation of isoprene-degrading bacteria in an estuarine environment |
| Author(s): | Johnston A; Crombie AT; El Khawand M; Sims L; Whited GM; McGenity TJ; Colin Murrell J; |
| Address: | "School of Environmental Science, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK. DuPont Industrial Biosciences, 925 Page Mill Road, Palo Alto, CA 94304, USA. School of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, CO4 3SQ, UK. Earth and Life Systems Alliance, Norwich Research Park, Norwich, UK" |
| ISSN/ISBN: | 1462-2920 (Electronic) 1462-2912 (Print) 1462-2912 (Linking) |
| Abstract: | "Approximately one-third of volatile organic compounds (VOCs) emitted to the atmosphere consists of isoprene, originating from the terrestrial and marine biosphere, with a profound effect on atmospheric chemistry. However, isoprene provides an abundant and largely unexplored source of carbon and energy for microbes. The potential for isoprene degradation in marine and estuarine samples from the Colne Estuary, UK, was investigated using DNA-Stable Isotope Probing (DNA-SIP). Analysis at two timepoints showed the development of communities dominated by Actinobacteria including members of the genera Mycobacterium, Rhodococcus, Microbacterium and Gordonia. Representative isolates, capable of growth on isoprene as sole carbon and energy source, were obtained from marine and estuarine locations, and isoprene-degrading strains of Gordonia and Mycobacterium were characterised physiologically and their genomes were sequenced. Genes predicted to be required for isoprene metabolism, including four-component isoprene monooxygenases (IsoMO), were identified and compared with previously characterised examples. Transcriptional and activity assays of strains growing on isoprene or alternative carbon sources showed that growth on isoprene is an inducible trait requiring a specific IsoMO. This study is the first to identify active isoprene degraders in estuarine and marine environments using DNA-SIP and to characterise marine isoprene-degrading bacteria at the physiological and molecular level" |
| Keywords: | "Base Sequence Butadienes/*metabolism Environment Genome, Bacterial/genetics Gordonia Bacterium/classification/genetics/*metabolism Hemiterpenes/*metabolism Mixed Function Oxygenases/genetics/*metabolism Mycobacterium/classification/genetics/*metabolism Pe;" |
| Notes: | "MedlineJohnston, Antonia Crombie, Andrew T El Khawand, Myriam Sims, Leanne Whited, Gregg M McGenity, Terry J Colin Murrell, J eng England 2017/06/28 Environ Microbiol. 2017 Sep; 19(9):3526-3537. doi: 10.1111/1462-2920.13842. Epub 2017 Jul 21" |
