| Title: | Characterization of volatile components of Iranian saffron using factorial-based response surface modeling of ultrasonic extraction combined with gas chromatography-mass spectrometry analysis |
| Author(s): | Jalali-Heravi M; Parastar H; Ebrahimi-Najafabadi H; |
| Address: | "Chemistry Department, Sharif University of Technology, Tehran, Iran. jalali@sharif.edu" |
| DOI: | 10.1016/j.chroma.2009.06.067 |
| ISSN/ISBN: | 1873-3778 (Electronic) 0021-9673 (Linking) |
| Abstract: | "The volatile components of Iranian saffron were extracted using ultrasonic solvent extraction (USE) technique and then were separated and detected by gas chromatography-mass spectrometry (GC-MS). Variables affecting the extraction procedure were screened by using a 2(5-1) fractional factorial design and among them; sample amount, solvent volume, solvent ratio and extraction time were optimized by applying a rotatable central composite design (CCD). The optimum values of factors were: 2.38g sample, 29.04mL solvent, 69.23% MeOH solvent ratio and 71.8min for the extraction time. Forty constituents were identified for Iranian saffron by GC-MS representing 90% of the total peak area. The major components were 2,6,6-trimethyl-1,3-cyclohexadiene-1-carboxaldehyde, namely safranal (26.29%), bicyclo[3,2,0]hept-2-ene-,4-ethoxy-,endo (5.69%), linoleic acid (4.77%), 4-hydroxy-2,6,6-trimethyl-1-cyclohexene-1-carboxaldehyde, namely HTCC (4.44%), and nonadecanol (3.32%). Some new compounds were identified for the first time in saffron. In addition, the results of this study were compared with those of Greek saffron" |
| Keywords: | Crocus/*chemistry Cyclohexenes/analysis Flowers/*chemistry Gas Chromatography-Mass Spectrometry Greece Iran Plant Extracts/chemistry/*isolation & purification Solvents Terpenes/analysis Ultrasonics Volatile Organic Compounds/*analysis; |
| Notes: | "MedlineJalali-Heravi, Mehdi Parastar, Hadi Ebrahimi-Najafabadi, Heshmatollah eng Netherlands 2009/07/15 J Chromatogr A. 2009 Aug 14; 1216(33):6088-97. doi: 10.1016/j.chroma.2009.06.067. Epub 2009 Jun 30" |
