| Title: | Interaction between mating-type proteins from the homothallic fungus Sordaria macrospora |
| Author(s): | Jacobsen S; Wittig M; Poggeler S; |
| Address: | "Department for General and Molecular Botany, Ruhr-University Bochum, Germany" |
| DOI: | 10.1007/s00294-002-0276-0 |
| ISSN/ISBN: | 0172-8083 (Print) 0172-8083 (Linking) |
| Abstract: | "Mating-type genes control sexual development in ascomycetes. Little is known about their function in homothallic species, which are self-fertile and do not require a mating partner for sexual reproduction. The function of mating-type genes in the homothallic fungus Sordaria macrospora was assayed using a yeast system in order to find properties typical of eukaryotic transcription factors. We were able to demonstrate that the mating-type proteins SMTA-1 and SMTa-1 have domains capable of activating transcription of yeast reporter genes. Two-hybrid analysis for heterodimerization and homodimerization revealed the ability of SMTA-1 to interact with SMTa-1 and vice versa. These two proteins are encoded by different mating types in the related heterothallic species Neurospora crassa. The interaction between SMTA-1 and SMTa-1 was defined by experiments with truncated versions of SMTA-1 and in vitro by means of protein cross-linking. Moreover, we gained evidence for homodimerization of SMTA-1. Possible functions of mating-type proteins in the homothallic ascomycete S. macrospora are discussed" |
| Keywords: | "Amino Acid Sequence Base Sequence DNA Primers Fungal Proteins/chemistry/genetics/*metabolism Genes, Fungal Genes, Mating Type, Fungal Mating Factor Molecular Sequence Data Peptides/chemistry/genetics/*metabolism Polymerase Chain Reaction Sequence Homology;" |
| Notes: | "MedlineJacobsen, Sabine Wittig, Michael Poggeler, Stefanie eng Research Support, Non-U.S. Gov't 2002/07/12 Curr Genet. 2002 Jun; 41(3):150-8. doi: 10.1007/s00294-002-0276-0. Epub 2002 May 28" |
