| Title: | Identification and characterization of three nearly identical linalool/nerolidol synthase from Acorus calamus |
| Author(s): | Ibdah M; Hino S; Nawade B; Yahyaa M; Bosamia TC; Shaltiel-Harpaz L; |
| Address: | "Newe Yaar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat, Israel. Electronic address: mwafaq@volcani.agri.gov.il. Newe Yaar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat, Israel. CSIR-Central Salt and Marine Chemical Research Institute, Bhavnagar Gujarat, India. Migal Galilee Research Institute, P.O. Box 831, Kiryat Shmona, 11016, Israel; Tel Hai College, Environmental Sciences Department, Upper Galilee, 12210, Israel" |
| DOI: | 10.1016/j.phytochem.2022.113318 |
| ISSN/ISBN: | 1873-3700 (Electronic) 0031-9422 (Linking) |
| Abstract: | "Acorus calamus is a perennial aromatic medicinal plant from the Acorusaceae family, known for its pharmaceutical and medicinal value. A combined chemical, biochemical, and molecular study was conducted to evaluate the differential accumulation of volatile organic compounds (VOCs) in rhizomes and leaves of A. calamus essential oil. Here, we performed VOC profiling and transcriptome-based identification and functional characterization of terpene synthase (TPS) genes. A total of 110 VOCs were detected from the rhizomes and leaves of A. calamus, and some VOCs showed significant differences between them. The further transcriptome-based analysis led to the identification of six putative TPSs genes. In phylogenetic analysis, three TPSs belonged to the TPS-g clade, one to each of the TPS-a, TPS-c, and TPS-e clades. The heterologous E. coli-based expression of recombinant TPSs identified three genes (AcTPS3, AcTPS4, and AcTPS5) as bifunctional linalool/nerolidol synthase. The correlation of TPS gene expression and VOC metabolite profiles supported the function of these genes in A. calamus. Our findings provide a roadmap for future efforts to enhance the molecular mechanisms of terpene biosynthesis and our understanding of Acorus-insect interactions" |
| Keywords: | "*Acorus/chemistry Acyclic Monoterpenes *Alkyl and Aryl Transferases/genetics Escherichia coli/metabolism *Oils, Volatile/chemistry Phylogeny Sesquiterpenes *Volatile Organic Compounds/metabolism Acorus calamus Acorusaceae Chemical compositions Terpene syn;" |
| Notes: | "MedlineIbdah, Mwafaq Hino, Shada Nawade, Bhagwat Yahyaa, Mosaab Bosamia, Tejas C Shaltiel-Harpaz, Liora eng England 2022/07/26 Phytochemistry. 2022 Oct; 202:113318. doi: 10.1016/j.phytochem.2022.113318. Epub 2022 Jul 21" |
