| Title: | Hepatitis B Surface Antigen S Gene is an Effective Carrier Molecule for Developing GnRH DNA Immunocastration Vaccine in Mice |
| Author(s): | Han YG; Ye WJ; Liu GQ; Jiang XP; Ijaz N; Zhao JY; Tesema B; |
| Address: | "Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China. Chongqing Key Laboratory of Forage & Herbivore, Chongqing Engineering Research Centre for Herbivores Resource Protection and Utilization, College of Animal Science and Technology, Southwest University, Chongqing, China" |
| ISSN/ISBN: | 1439-0531 (Electronic) 0936-6768 (Linking) |
| Abstract: | "Relatively molecular mass of GnRH antigens is small and hence needs to couple to a large carrier molecule to enhance its immunogenicity. This study investigated whether hepatitis B surface antigen S (HBsAg-S) gene can be used as an effective carrier molecule for developing GnRH DNA immunocastration vaccine. Two copies of human GnRH gene were fused with HBsAg-S gene for constructing a recombinant plasmid pVAX-HBsAg-S-2GnRH that coded for 27 kDa target fusion protein. Ten male mice were divided into two equal groups, treatment and control. The vaccine (50 mug/mice) prepared in saline solution was injected into male mice at weeks 0, 1, 2, 4 and 7 of the experiment. Vaccine's efficacy was evaluated in terms of GnRH-specific IgG antibody response, plasma testosterone levels, testicular weight and extent of the testicular tissue damage. The specific anti-GnRH antibody titre in vaccinated animals was significantly higher than in controls in only 4th week of immunization (p < 0.05). In addition, vaccinated animals showed lower testicular weight than those of the controls (p < 0.05). Spermatogenesis in seminiferous tubules in vaccinated animals was suppressed. In conclusion, in this study, the engineered plasmid to be used as a GnRH DNA vaccine induced antibody response and suppressed spermatogenesis in mice. This suggests that HBsAg-S gene can be an effective carrier molecule for developing GnRH DNA immunocastration vaccine when relatively molecular mass of the aimed antigens is small" |
| Keywords: | "Animals Gonadotropin-Releasing Hormone/genetics/*immunology Hepatitis B Surface Antigens/*genetics Humans Immunization Immunoglobulin G/blood Male Mice Organ Size Recombinant Fusion Proteins/genetics/*immunology Spermatogenesis/*immunology Sterilization, ;" |
| Notes: | "MedlineHan, Y G Ye, W J Liu, G Q Jiang, X P Ijaz, N Zhao, J Y Tesema, B eng Germany 2016/05/10 Reprod Domest Anim. 2016 Jun; 51(3):445-50. doi: 10.1111/rda.12692" |
