« Previous Abstract"Identification of Components of the Aggregation Pheromone of the Guam Strain of Coconut Rhinoceros Beetle, Oryctes rhinoceros, and Determination of Stereochemistry"    Next Abstract"Midgut tissue of male pine engraver, Ips pini, synthesizes monoterpenoid pheromone component ipsdienol de novo" »

Insect Biochem Mol Biol

Title:		"Male jeffrey pine beetle, Dendroctonus jeffreyi, synthesizes the pheromone component frontalin in anterior midgut tissue"
Author(s):		Hall GM; Tittiger C; Blomquist GJ; Andrews GL; Mastick GS; Barkawi LS; Bengoa C; Seybold SJ;
Address:		"Department of Biochemistry/MS 330, University of Nevada, Reno, NV 89557-0014, USA"
Journal Title:		Insect Biochem Mol Biol
Year:		2002
Volume:		32
Issue:		11
Page Number:		1525 - 1532
DOI:		10.1016/s0965-1748(02)00073-5
ISSN/ISBN:		0965-1748 (Print) 0965-1748 (Linking)
Abstract:		"The male Jeffrey pine beetle, Dendroctonus jeffreyi Hopkins (Coleoptera: Scolytidae), produces the bicyclic ketal frontalin as part of a complex semiochemical blend. A key regulated enzyme in the mevalonate pathway, 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-R), showed high transcript levels in the anterior midgut of male Jeffrey pine beetles by in situ hybridization. HMG-R expression in this area of the alimentary canal was related to male emergence, where emerged males demonstrated significant up-regulation of HMG-R transcript and pre-emerged males showed only basal levels. Pre-emerged males were induced to express high levels of HMG-R transcript by treatment with juvenile hormone (JH) III. Additionally, isolated anterior midgut tissue from JH III-treated males converted radiolabeled acetate to frontalin, as assayed by radio-HPLC, providing strong evidence that this is the site of frontalin production in male beetles"
Keywords:		"Acetates/metabolism Animals Bridged Bicyclo Compounds, Heterocyclic/*metabolism Coleoptera/anatomy & histology/genetics/*physiology *Digestive System Physiological Phenomena Organ Specificity Pheromones/*biosynthesis Pinus/*parasitology;"
Notes:		"MedlineHall, G M Tittiger, C Blomquist, G J Andrews, G L Mastick, G S Barkawi, L S Bengoa, C Seybold, S J eng R01 HD038069/HD/NICHD NIH HHS/ Research Support, U.S. Gov't, Non-P.H.S. England 2003/01/18 Insect Biochem Mol Biol. 2002 Nov; 32(11):1525-32. doi: 10.1016/s0965-1748(02)00073-5"