| Title: | Comparison of Artemisiae argyi Folium and Artemisiae lavandulaefoliae Folium by simultaneous determination of multi-components with single reference standard method and chemometric analysis |
| Author(s): | Guo L; Zhang D; Xue ZJ; Jiao Q; Liu AP; Zheng YG; Liu EH; Duan L; |
| Address: | "School of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang, Hebei, P. R. China. Hebei Key Laboratory of Chinese Medicine Research on Cardio-cerebrovascular Disease, Hebei University of Chinese Medicine, Shijiazhuang, Hebei, P. R. China. State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, P. R. China. College of Chemistry and Material Science, Hebei Normal University, Shijiazhuang, Hebei, P. R. China" |
| ISSN/ISBN: | 1099-1565 (Electronic) 0958-0344 (Linking) |
| Abstract: | "INTRODUCTION: Artemisiae argyi Folium and Artemisiae lavandulaefoliae Folium, two morphologically similar herbal medicines derived from Artemisia genus. Although the two Artemisia herbs have been used as medicines for a long time in China, the study of their phytochemical and bioactive composition is limited. OBJECTIVE: To comprehensively compare and evaluate the composition of Artemisiae argyi Folium and Artemisiae lavandulaefoliae Folium, and find the chemical makers for quality evaluation of the two Artemisia herbal medicines. METHODOLOGY: Eight compounds including six phenolic acids and two flavonoids were assayed by a single reference standard for simultaneous determination of multi-components method using 3-caffeoylquinic acid as the reference standard. The quantitative data were further analysed by chemometric approaches to compare and distinguish the two herbal medicines. RESULTS: The credibility and feasibility of the single reference standard for simultaneous determination of the multi-components method were carefully validated. The validated method was applied to analyse 16 batches of Artemisiae argyi Folium and 10 batches of Artemisiae lavandulaefoliae Folium samples. The quantitative results showed that 3,5-di-O-caffeoylquinic acid was the most abundant constituent, and the contents of flavonoids were notably different between the two herbal medicines. The chemometric analysis results indicated the two flavonoids, jaceosidin and eupatilin could be used as chemical markers for quality evaluation of the two herbal medicines. CONCLUSION: The single reference standard for simultaneous determination of the multi-components method coupled with chemometrics analysis could be a well-acceptable strategy to compare and evaluate the quality of Artemisiae argyi Folium and Artemisiae lavandulaefoliae Folium" |
| Keywords: | "Artemisia/*chemistry/classification Chromatography, High Pressure Liquid/*methods Cluster Analysis Drugs, Chinese Herbal/*chemistry/*standards Limit of Detection Principal Component Analysis Quality Control Reference Standards Species Specificity Spectrop;" |
| Notes: | "MedlineGuo, Long Zhang, Dan Xue, Zi-Jing Jiao, Qian Liu, Ai-Peng Zheng, Yu-Guang Liu, E-Hu Duan, Li eng L2017B11/Science Foundation of Hebei Normal University/ BSZ2017003/Science Foundation of Hebei University of Chinese Medicine/ 2018108/Research Foundation of Hebei Provincial Administration of Traditional Chinese Medicine/ QN2016103/Research Foundation of Hebei Province Education Department/ QN2017093/Research Foundation of Hebei Province Education Department/ H2018205185/Natural Science Foundation of Hebei Province/ H2018423032/Natural Science Foundation of Hebei Province/ Comparative Study Validation Study England 2018/09/27 Phytochem Anal. 2019 Jan; 30(1):14-25. doi: 10.1002/pca.2786. Epub 2018 Sep 5" |
