| Title: | Neurospora crassa A mating-type region |
| Author(s): | Glass NL; Grotelueschen J; Metzenberg RL; |
| Address: | "Department of Physiological Chemistry, University of Wisconsin, Madison 53706" |
| ISSN/ISBN: | 0027-8424 (Print) 1091-6490 (Electronic) 0027-8424 (Linking) |
| Abstract: | "The mating-type locus of the haploid filamentous fungus Neurospora crassa is a regulatory region that controls entry into the sexual cycle and prevents formation of mixed mating-type heterokaryons in the vegetative phase. The locus consists of alternative sequences called A and a. The A mating-type DNA sequence of Neurospora crassa is composed of a region of 5301 base pairs that has little similarity to the sequence present at the mating-type locus in an a mating-type strain. However, the sequences flanking the mating-type locus in the A haploid and a haploid genome are essentially identical. The region of the A mating-type sequence required for expression of the heterokaryon incompatibility and sexual functions has been localized to a single open reading frame (ORF) encoding a polypeptide of 288 amino acids. Sequence analysis of sterile, heterokaryon-compatible mutants reveals frameshift mutations in this same ORF. The putative 288-amino acid product has a region of similarity to the MAT alpha 1 polypeptide of Saccharomyces cerevisiae" |
| Keywords: | "Amino Acid Sequence Base Sequence Chromosome Deletion Cloning, Molecular Escherichia coli/genetics Gene Amplification *Genes, Fungal *Genes, Mating Type, Fungal Haploidy Mating Factor Molecular Sequence Data Neurospora/*genetics Neurospora crassa/*genetic;" |
| Notes: | "MedlineGlass, N L Grotelueschen, J Metzenberg, R L eng GM 08995/GM/NIGMS NIH HHS/ Comparative Study Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1990/07/01 Proc Natl Acad Sci U S A. 1990 Jul; 87(13):4912-6. doi: 10.1073/pnas.87.13.4912" |
