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J Sep Sci

Title:		Headspace solid-phase microextraction combined with GCxGC-TOFMS for the analysis of volatile compounds of Coptis species rhizomes
Author(s):		Gao X; Yang X; Mitrevski BS; Marriott PJ;
Address:		"State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Health Science Centre, Peking University, Beijing, P. R. China"
Journal Title:		J Sep Sci
Year:		2011
Volume:		20110413
Issue:		10
Page Number:		1157 - 1166
DOI:		10.1002/jssc.201100022
ISSN/ISBN:		1615-9314 (Electronic) 1615-9306 (Linking)
Abstract:		"In this study, the investigation of the volatile compounds of dried rhizomes of Coptis chinensis Franch, C. deltoidea C. Y. Cheng et Hsiao, and C. teeta Wall was carried out to complete the chemical composition of these valuable natural products. Volatile profiles were established and compared after headspace solid-phase microextraction (HS-SPME) using a polydimethylsiloxane/divinylbenzene (PDMS/DVB, 65 mum) fibre coupled to comprehensive 2D gas chromatography time-of-flight mass spectrometry (GCxGC-TOFMS). Analyses were performed and compared on two column-phase combinations (non-polar/polar and polar/non-polar). The majority of the identified compounds eluted as well-separated (pure) components as a result of high-resolution capability of the GCxGC method, which significantly reduces co-elution. Therefore, this increases the likelihood that pure mass spectra can be obtained. More than 290 volatile and semi-volatile organic compounds were tentatively characterized by means of GCxGC in tandem with TOFMS detection. Improved result interpretations were obtained in terms of compound classification based on the organized structure of the peaks of structurally related compounds in the GCxGC contour plot. These compounds are distributed over the chemical groups of hydrocarbons, acids, alkenes, alkynes, aldehydes, ketones, alcohols, esters, furans, and terpenoids. Among all the chemical groups, terpenoids present the higher number of identified compounds (44), alkenes (41), and aldehydes and ketones (28). This study completed the volatile phytochemical analysis of the headspace composition of various Coptis species rhizomes, and should serve as a means to identify the difference between the rhizomes and may also be useful to confirm individual species based on their volatile chemical profile"
Keywords:		"Coptis/*chemistry Drugs, Chinese Herbal/*chemistry/*isolation & purification Gas Chromatography-Mass Spectrometry/*methods Solid Phase Microextraction/*methods Volatile Organic Compounds/*chemistry/*isolation & purification;"
Notes:		"MedlineGao, Xin Yang, Xiuwei Mitrevski, Blagoj S Marriott, Philip J eng Evaluation Study Research Support, Non-U.S. Gov't Germany 2011/04/15 J Sep Sci. 2011 May; 34(10):1157-66. doi: 10.1002/jssc.201100022. Epub 2011 Apr 13"