| Title: | Completion of the nucleotide sequence of the Enterococcus faecalis conjugative virulence plasmid pAD1 and identification of a second transfer origin |
| Author(s): | Francia MV; Haas W; Wirth R; Samberger E; Muscholl-Silberhorn A; Gilmore MS; Ike Y; Weaver KE; An FY; Clewell DB; |
| Address: | "Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, MI 48109, USA" |
| ISSN/ISBN: | 0147-619X (Print) 0147-619X (Linking) |
| Abstract: | "pAD1 is a 59.3-kb plasmid in Enterococcus faecalis that has been the subject of intense investigation with regard to its pheromone-inducible conjugation behavior as well as its contribution to virulence. Approximately two-thirds of the pAD1 nucleotide sequence has been previously reported. Here we report on an analysis of the final approximately 22 kb, a significant portion of which is believed to encode structural genes associated with conjugation. The conjugation-related region was also found to contain a new (second) origin of conjugative transfer (oriT). A list of open reading frames covering the entire plasmid is presented" |
| Keywords: | "Conjugation, Genetic/*genetics Deoxyribonuclease EcoRI/metabolism Enterococcus faecalis/*genetics/*pathogenicity Genes, Bacterial/*genetics Molecular Sequence Data Open Reading Frames/genetics Plasmids/*genetics Regulatory Sequences, Nucleic Acid/genetics;" |
| Notes: | "MedlineFrancia, M V Haas, W Wirth, R Samberger, E Muscholl-Silberhorn, A Gilmore, M S Ike, Y Weaver, K E An, F Y Clewell, D B eng AI40963/AI/NIAID NIH HHS/ AI41108/AI/NIAID NIH HHS/ EY12190/EY/NEI NIH HHS/ GM33956/GM/NIGMS NIH HHS/ MO1-RR00042/RR/NCRR NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 2001/10/10 Plasmid. 2001 Sep; 46(2):117-27. doi: 10.1006/plas.2001.1533" |
