« Previous AbstractAlgae as a Source of Natural Flavors in Innovative Foods    Next Abstract"Investigating aroma diversity combining purge-and-trap, comprehensive two-dimensional gas chromatography, and mass spectrometry" »

Anal Chim Acta

Title:		Evaluation of different adsorbent materials for the untargeted and targeted bacterial VOC analysis using GCxGC-MS
Author(s):		Franchina FA; Purcaro G; Burklund A; Beccaria M; Hill JE;
Address:		"Thayer School of Engineering at Dartmouth College, 03755, Hanover, NH, United States. Thayer School of Engineering at Dartmouth College, 03755, Hanover, NH, United States. Electronic address: jane.hill@dartmouth.edu"
Journal Title:		Anal Chim Acta
Year:		2019
Volume:		20190322
Issue:
Page Number:		146 - 153
DOI:		10.1016/j.aca.2019.03.027
ISSN/ISBN:		1873-4324 (Electronic) 0003-2670 (Linking)
Abstract:		"The analysis of bacterial volatile organic compounds has gained attraction as a non-invasive way to identify disease-causing organisms, given that bacteria have unique metabolisms and volatile metabolic byproducts. In the present research, different adsorbent materials (Carbopack Y, X, B, Carboxen 1000 and Tenax TA), packed singularly or in combination, were compared in terms of sampling performance (sensitivity, repeatability and selectivity) for the extraction of standards and bacterial volatile metabolites in vitro (from Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli). After extraction, bacterial volatile organic compounds were desorbed and analyzed in a comprehensive two-dimensional gas chromatography system coupled to a time-of-flight mass spectrometer (GC?ª+x?ª+GC-ToF MS). The results show that Tenax has the greater ability to extract the standard mix as well as volatile organic compounds with better repeatability (4-26 RSD%), higher sensitivity (on average approximately 24 fold) compared to Carbopack Y, X and Carboxen 1000 tube, which followed in terms of performance. In addition, Tenax confirmed the best sensitivity and discriminatory power with no misclassification in the untargeted and unsupervised analysis for the differentiation of the bacterial species"
Keywords:		"*Adsorption Chromatography, Gas Escherichia coli/*chemistry Mass Spectrometry Pseudomonas aeruginosa/*chemistry Staphylococcus aureus/*chemistry Surface Properties Volatile Organic Compounds/*analysis Bacterial metabolites Comprehensive two-dimensional ga;"
Notes:		"MedlineFranchina, Flavio A Purcaro, Giorgia Burklund, Alison Beccaria, Marco Hill, Jane E eng R56 HL139846/HL/NHLBI NIH HHS/ Netherlands 2019/04/28 Anal Chim Acta. 2019 Aug 20; 1066:146-153. doi: 10.1016/j.aca.2019.03.027. Epub 2019 Mar 22"