| Title: | "DREADD-Induced Silencing of the Medial Olfactory Tubercle Disrupts the Preference of Female Mice for Opposite-Sex Chemosignals(1,2,3)" |
| Author(s): | DiBenedictis BT; Olugbemi AO; Baum MJ; Cherry JA; |
| Address: | "Department of Biology, Boston University , Boston, Massachusetts 02215. Department of Psychological and Brain Sciences, Boston University , Boston, Massachusetts 02215" |
| DOI: | 10.1523/ENEURO.0078-15.2015 |
| ISSN/ISBN: | 2373-2822 (Print) 2373-2822 (Electronic) 2373-2822 (Linking) |
| Abstract: | "Attraction to opposite-sex pheromones during rodent courtship involves a pathway that includes inputs to the medial amygdala (Me) from the main and accessory olfactory bulbs, and projections from the Me to nuclei in the medial hypothalamus that control reproduction. However, the consideration of circuitry that attributes hedonic properties to opposite-sex odors has been lacking. The medial olfactory tubercle (mOT) has been implicated in the reinforcing effects of natural stimuli and drugs of abuse. We performed a tract-tracing study wherein estrous female mice that had received injections of the retrograde tracer, cholera toxin B, into the mOT were exposed to volatile odors from soiled bedding. Both the anterior Me and ventral tegmental area sent direct projections to the mOT, of which a significant subset was selectively activated (expressed Fos protein) by testes-intact male (but not female) volatile odors from soiled bedding. Next, the inhibitory DREADD (designer receptors exclusively activated by designer drugs) receptor hM4Di was bilaterally expressed in the mOT of female mice. Urinary preferences were then assessed after intraperitoneal injection of either saline or clozapine-N-oxide (CNO), which binds to the hM4Di receptor to hyperpolarize infected neurons. After receiving CNO, estrous females lost their preference for male over female urinary odors, whereas the ability to discriminate these odors remained intact. Male odor preference returned after vehicle treatment in counterbalanced tests. There were no deficits in locomotor activity or preference for food odors when subject mice received CNO injections prior to testing. The mOT appears to be a critical segment in the pheromone-reward pathway of female mice" |
| Keywords: | Dreadd hM4Di olfactory tubercle retrograde tract tracing; |
| Notes: | "PubMed-not-MEDLINEDiBenedictis, Brett T Olugbemi, Adaeze O Baum, Michael J Cherry, James A eng R01 DC008962/DC/NIDCD NIH HHS/ 2015/10/20 eNeuro. 2015 Sep 22; 2(5):ENEURO.0078-15.2015. doi: 10.1523/ENEURO.0078-15.2015. eCollection 2015 Sep" |
