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J Chromatogr A

Title:		Analysis of volatile bases by high performance liquid chromatography with aerosol-based detection
Author(s):		Cohen RD; Liu Y; Gong X;
Address:		"Merck Research Laboratories, Merck & Co., Inc., Rahway, NJ 07065, USA. ryan.cohen@merck.com"
Journal Title:		J Chromatogr A
Year:		2012
Volume:		20120120
Issue:
Page Number:		172 - 179
DOI:		10.1016/j.chroma.2012.01.036
ISSN/ISBN:		1873-3778 (Electronic) 0021-9673 (Linking)
Abstract:		"The development and performance of two methods are described whereby low levels of volatile bases are quantified by HPLC using either a charged aerosol detector (CAD) or a nano-quantity analyte detector (NQAD). A test set of 12 volatile bases (ammonia, hydrazine, methylamine, ethylamine, diethylamine, triethylamine, isobutylamine, N,N-diisopropylethylamine, morpholine, piperazine, ethylenediamine, and 1,4-diazabicyclo[2.2.2]octane) were analyzed. The studied compounds all exhibit poor UV chromophores and are typically undetectable by aerosol-based detection when using conventional volatile mobile phases. The ability to detect these analytes by CAD or NQAD depended on their propensity towards formation of a low volatility salt between the target analyte and mobile phase modifier. Trifluoroacetic acid (TFA) was found to significantly improve detection of most volatile bases. A low concentration (0.2 mM) of hydrochloric acid was additionally needed to enable detection of ammonia. The compounds were separated under hydrophilic interaction liquid chromatography (HILIC) conditions on a zic-pHILIC column. For all analytes, limits of detection (LOD) were measured in the range of 1-27 ng on column, which is comparable to previously reported detection limits for non-volatile analytes"
Keywords:		"Aerosols/*chemistry Amines/analysis Ammonia/analysis Chromatography, High Pressure Liquid/*methods Hydrazines/analysis Hydrochloric Acid Hydrophobic and Hydrophilic Interactions Limit of Detection Nanotechnology Piperazine Piperazines/analysis Trifluoroac;"
Notes:		"MedlineCohen, Ryan D Liu, Yong Gong, Xiaoyi eng Research Support, Non-U.S. Gov't Netherlands 2012/02/07 J Chromatogr A. 2012 Mar 16; 1229:172-9. doi: 10.1016/j.chroma.2012.01.036. Epub 2012 Jan 20"