| Title: | Transcriptome analysis reveals the effects of grafting on sugar and alpha-linolenic acid metabolisms in fruits of cucumber with two different rootstocks |
| Author(s): | Zhao L; Liu A; Song T; Jin Y; Xu X; Gao Y; Ye X; Qi H; |
| Address: | "College of Horticulture, Shenyang Agricultural University/Key Laboratory of Protected Horticulture, Ministry of Education/Northern National& Local Joint Engineering Research Center of Horticultural Facilities Design and Application Technology(Liaoning), Shenyang 110866, China; Institute of Vegetable Research, Liaoning Academy of Agricultural Sciences, Shenyang, Liaoning 110866, China. Electronic address: 44992698@qq.com. Institute of Vegetable Research, Liaoning Academy of Agricultural Sciences, Shenyang, Liaoning 110866, China. Electronic address: laq2004@sohu.com. Institute of Vegetable Research, Liaoning Academy of Agricultural Sciences, Shenyang, Liaoning 110866, China. Electronic address: songteifeng@126.com. College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, Heilong Jiang 163319, China. Electronic address: 277168601@qq.com. College of Horticulture, Shenyang Agricultural University/Key Laboratory of Protected Horticulture, Ministry of Education/Northern National& Local Joint Engineering Research Center of Horticultural Facilities Design and Application Technology(Liaoning), Shenyang 110866, China. Electronic address: 1446001934@qq.com. Agricultural Technology Extension Centre of Changtu' Country, Tieling, Liaoning 112599, China. Electronic address: ctxtfz@126.com. College of Horticulture, Shenyang Agricultural University/Key Laboratory of Protected Horticulture, Ministry of Education/Northern National& Local Joint Engineering Research Center of Horticultural Facilities Design and Application Technology(Liaoning), Shenyang 110866, China. Electronic address: yxl050112@163.com. College of Horticulture, Shenyang Agricultural University/Key Laboratory of Protected Horticulture, Ministry of Education/Northern National& Local Joint Engineering Research Center of Horticultural Facilities Design and Application Technology(Liaoning), Shenyang 110866, China. Electronic address: hyqiaaa@126.com" |
| DOI: | 10.1016/j.plaphy.2018.07.008 |
| ISSN/ISBN: | 1873-2690 (Electronic) 0981-9428 (Linking) |
| Abstract: | "Flavor quality in cucumber is affected by different rootstocks, but the molecular mechanism is largely unclean. To clarify the differences of sugar and aromatic compounds, cucumber (cucumis sativus) fruits from plants of self-grafted (SG) or grafted onto figleaf gourd (Cucurbita ficifolia; G1) or 'Weisheng No.1' rootstock (Cucurbita moschata ?a?Cucurbita moschata hybrids; G2) were performed the transcriptome analysis. We obtained 1013 and 920 differentially expressed genes (DEGs) from G1 and G2 compared to SG respectively, in which 453 genes were co-expressed. Functional annotations showed many DEGs were involved in glycolysis/gluconeogenesis metabolism, fructose metabolism and alpha-Linolenic acid metabolisms, 20 DEGs were selected from the 3 pathways to validate sequencing accuracy by quantitative real-time PCR. The gene relative expression levels were concurrent with RNA-seq results and sugar and aromatic compounds content phenotypes. Moreover, some vital transcript factors and transport proteins were analyzed. These findings indicate that different rootstocks could induce significantly changes in the physiological profiling and transcripts of sugar- and aromatic flavor-related genes. This study provides a novel insight into the molecular mechanisms of fruit quality regulated by candidate genes" |
| Keywords: | "Cucumis sativus/genetics/*metabolism/physiology Fructose/metabolism Fruit/*metabolism Gene Expression Profiling Gene Expression Regulation, Plant Plant Roots/*metabolism/physiology Real-Time Polymerase Chain Reaction Sugars/*metabolism Volatile Organic Co;" |
| Notes: | "MedlineZhao, Lili Liu, Aiqun Song, Tiefeng Jin, Yazhong Xu, Xin Gao, Yang Ye, Xueling Qi, Hongyan eng France 2018/07/24 Plant Physiol Biochem. 2018 Sep; 130:289-302. doi: 10.1016/j.plaphy.2018.07.008. Epub 2018 Jul 20" |
