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Plant Physiol Biochem

Title:		"Transcription profile analysis of Lycopersicum esculentum leaves, unravels volatile emissions and gene expression under salinity stress"
Author(s):		Zhang J; Zeng L; Chen S; Sun H; Ma S;
Address:		"Department of Chemical Engineering, Xiangtan University, Xiangtan 411105, China. Electronic address: jihongzh01@xtu.edu.cn. Department of Chemical Engineering, Xiangtan University, Xiangtan 411105, China"
Journal Title:		Plant Physiol Biochem
Year:		2018
Volume:		20180217
Issue:
Page Number:		11 - 21
DOI:		10.1016/j.plaphy.2018.02.016
ISSN/ISBN:		1873-2690 (Electronic) 0981-9428 (Linking)
Abstract:		"Salinity stress can impede development and plant growth adversely. However, there is very little molecular information on NaCl resistance and volatile emissions in Lycopersicum esculentum. In order to investigate the effects of salt stress on the release of volatile compounds, we quantified and compared transcriptome changes by RNA-Seq analysis and volatile constituents with gas chromatography/mass spectrometry (GC/MS) coupled with solid-phase microextraction (SPME) after exposure to continuous salt stress. Chemical analysis by GC-MS analysis revealed that NaCl stress had changed species and quantity of volatile compounds released. In this research, 21,578 unigenes that represented 44,714 assembled unique transcripts were separated from tomato leaves exposed to NaCl stress based on de novo transcriptome assembly. The total number of differentially expressed genes was 7210 after exposure to NaCl, including 6200 down-regulated and 1208 up-regulated genes. Among these differentially expressed genes (DEGs), there were eighteen differentially expressed genes associated with volatile biosynthesis. Of the unigenes, 3454 were mapped to 131 KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways, mainly those are involved in RNA transport, plant-pathogen interactions, and plant hormone signal transduction. qRT-PCR analysis showed that NaCl exposure affected the expression profiles of the biosynthesis genes for eight volatile compounds (IPI, GPS, and TPS, etc.), which corresponded well with the RNA-Seq analysis and GC-MS results. Our results suggest that NaCl stress affects the emission of volatile substances from L. esculentum leaves by regulating the expression of genes that are involved in volatile organic compounds' biosynthesis"
Keywords:		"*Gene Expression Profiling *Gene Expression Regulation, Plant Solanum lycopersicum/*metabolism Oils, Volatile/*metabolism Plant Leaves/*metabolism *Salinity *Stress, Physiological Gc-ms Lycopersicum esculentum Salinity stress Transcriptome Volatile emissi;"
Notes:		"MedlineZhang, Jihong Zeng, Li Chen, Shaoyang Sun, Helong Ma, Shuang eng France 2018/02/27 Plant Physiol Biochem. 2018 May; 126:11-21. doi: 10.1016/j.plaphy.2018.02.016. Epub 2018 Feb 17"