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Plant Biotechnol J

Title:		"R2R3 MYB-dependent auxin signalling regulates trichome formation, and increased trichome density confers spider mite tolerance on tomato"
Author(s):		Yuan Y; Xu X; Luo Y; Gong Z; Hu X; Wu M; Liu Y; Yan F; Zhang X; Zhang W; Tang Y; Feng B; Li Z; Jiang CZ; Deng W;
Address:		"Key Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing University, Chongqing, China. Center of Plant Functional Genomics, Institute of Advanced Interdisciplinary Studies, Chongqing University, Chongqing, China. Department of Plant Sciences, University of California, Davis, CA, USA. College of Agriculture, Guangxi University, Nanning, China. Crops Pathology and Genetics Research Unit, United States Department of Agriculture, Agricultural Research Service, Davis, CA, USA"
Journal Title:		Plant Biotechnol J
Year:		2021
Volume:		20200726
Issue:		1
Page Number:		138 - 152
DOI:		10.1111/pbi.13448
ISSN/ISBN:		1467-7652 (Electronic) 1467-7644 (Print) 1467-7644 (Linking)
Abstract:		"Unicellular and multicellular tomato trichomes function as mechanical and chemical barriers against herbivores. Auxin treatment increased the formation of II, V and VI type trichomes in tomato leaves. The auxin response factor gene SlARF4, which was highly expressed in II, V and VI type trichomes, positively regulated the auxin-induced formation of II, V and VI type trichomes in the tomato leaves. SlARF4 overexpression plants with high densities of these trichomes exhibited tolerance to spider mites. Two R2R3 MYB genes, SlTHM1 and SlMYB52, were directly targeted and inhibited by SlARF4. SlTHM1 was specifically expressed in II and VI type trichomes and negatively regulated the auxin-induced formation of II and VI type trichomes in the tomato leaves. SlTHM1 down-regulation plants with high densities of II and VI type trichomes also showed tolerance to spider mites. SlMYB52 was specifically expressed in V type trichomes and negatively regulated the auxin-induced formation of V type trichome in the tomato leaves. The regulation of SlARF4 on the formation of II, V and VI type trichomes depended on SlTHM1 and SlMYB52, which directly targeted cyclin gene SlCycB2 and increased its expression. In conclusion, our data indicates that the R2R3 MYB-dependent auxin signalling pathway regulates the formation of II, V and VI type trichomes in tomato leaves. Our study provides an effective method for improving the tolerance of tomato to spider mites"
Keywords:		"Animals Gene Expression Regulation, Plant/genetics Indoleacetic Acids *Solanum lycopersicum/genetics Plant Leaves/genetics *Tetranychidae/genetics Trichomes Myb auxin response factor tomato transcription factor trichome;"
Notes:		"MedlineYuan, Yujin Xu, Xin Luo, Yingqing Gong, Zehao Hu, Xiaowei Wu, Mengbo Liu, Yudong Yan, Fang Zhang, Xiaolan Zhang, Wenfa Tang, Yuwei Feng, Bihong Li, Zhengguo Jiang, Cai-Zhong Deng, Wei eng Research Support, Non-U.S. Gov't England 2020/07/13 Plant Biotechnol J. 2021 Jan; 19(1):138-152. doi: 10.1111/pbi.13448. Epub 2020 Jul 26"